Gas N, Escande M L, Stevens B J
Biol Cell. 1985;53(3):209-18. doi: 10.1111/j.1768-322x.1985.tb00369.x.
The localization of a major nucleolar protein with a molecular weight of 100,000 has been followed during mitosis in Chinese hamster ovary CHO cells using specific antibodies to this protein and immunocytochemical techniques. The 100 kDa protein was visualized at discrete sites on metaphase chromosomes, corresponding to nucleolus organizer regions, and in large, immunostained nucleolar remnants that are discarded in the cytoplasm after nucleolar disintegration. After mitosis, the 100 kDa protein was shown to play an early role in nucleolar reformation. It was first detected in small deposits around the anaphase chromosomes. In telophase, the protein accumulated simultaneously in prenucleolar bodies and in the reforming nucleoli. The early presence of the 100 kDa protein in the telophase nucleus suggests that it is essential for the reestablishment of nucleolar function after mitosis. Thus this protein is present throughout the CHO cell cycle, an observation which supports the hypothesis that it plays a fundamental role in cell organization.
利用针对一种分子量为100,000的主要核仁蛋白的特异性抗体及免疫细胞化学技术，在中国仓鼠卵巢（CHO）细胞的有丝分裂过程中追踪了该蛋白的定位。在中期染色体上对应于核仁组织区的离散位点以及在核仁解体后被丢弃到细胞质中的大型免疫染色核仁残余物中，均可观察到100 kDa蛋白。有丝分裂后，100 kDa蛋白在核仁重新形成过程中发挥早期作用。它首先在后期染色体周围的小沉积物中被检测到。在末期，该蛋白同时在核仁前体和正在重新形成的核仁中积累。100 kDa蛋白在末期细胞核中的早期存在表明，它对于有丝分裂后核仁功能的重建至关重要。因此，这种蛋白在整个CHO细胞周期中均存在，这一观察结果支持了它在细胞组织中发挥基本作用的假说。
