Babkina G T, Karpova G G, Matasova N B
Mol Biol (Mosk). 1984 Sep-Oct;18(5):1287-96.
It was shown that Phe-tRNA Phe derivatives bearing arylazidogroups scattered statistically on N7 guanosine residues retain the ability to EF-Tu-dependent binding to E. coli ribosomes. UV-irradiation of the corresponding complex with the derivative of Phe-tRNA Phe located at A-site results in a specific modification of both ribosomal subunits to an approximately equal extent. It was found that proteins S9, S15, S16, S17, S18, S19 and L8/L9, L13, L15, L27 are labelled at A-site.
结果表明，携带芳基叠氮基团且随机分布于N7鸟苷残基上的苯丙氨酰 - tRNA苯丙氨酸衍生物保留了依赖延伸因子 - Tu与大肠杆菌核糖体结合的能力。用位于A位点的苯丙氨酰 - tRNA苯丙氨酸衍生物对相应复合物进行紫外线照射，会导致两个核糖体亚基发生程度大致相等的特异性修饰。研究发现，蛋白质S9、S15、S16、S17、S18、S19以及L8/L9、L13、L15、L27在A位点被标记。
