Vladimirov S N, Graifer D M, Karpova G G, Makhno V I, Kirillov S V
FEBS Lett. 1985 Feb 25;181(2):367-72. doi: 10.1016/0014-5793(85)80294-5.
From the affinity labelling of 70 S ribosomes with a photoreactive derivative of Phe-tRNAPhe bearing an arylazido group on guanine residues, it has been found that different sets of ribosomal proteins are labelled in the course of three successive steps of EF-Tu-dependent binding of aminoacyl-tRNA derivative at the A-site. Proteins S5, S7, S8, S16, S17, L9, L14, L15 and L24 were labelled before GTP hydrolysis; proteins S5, S7, S9, S11, S14, S18, S19, S21, L9, L21 and L29--after GTP hydrolysis; proteins S2, S5, S7, S21, L11 and L23--after GTP hydrolysis and transpeptidation.
通过用在鸟嘌呤残基上带有芳基叠氮基的苯丙氨酰 - tRNAphe的光反应性衍生物对70S核糖体进行亲和标记，发现在氨酰 - tRNA衍生物在A位点的EF - Tu依赖性结合的三个连续步骤中，有不同组的核糖体蛋白被标记。在GTP水解之前，蛋白质S5、S7、S8、S16、S17、L9、L14、L15和L24被标记；在GTP水解之后，蛋白质S5、S7、S9、S11、S14、S18、S19、S21、L9、L21和L29被标记；在GTP水解和转肽作用之后，蛋白质S2、S5、S7、S21、L11和L23被标记。
