Dambaugh T, Hennessy K, Chamnankit L, Kieff E
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7632-6. doi: 10.1073/pnas.81.23.7632.
Sequence analysis of the U2 regions of the B95-8 and AG876 Epstein-Barr virus (EBV) isolates reveals divergence within a long open reading frame previously identified as encoding 1.5 kilobases of the 3' end of a viral RNA expressed in latently infected, growth-transformed, B-lymphocyte cell lines. Differences among EBV isolates within the U2 open reading frame are shown to correlate with differences in an EBV nuclear antigen, EBNA2. B95-8, W91, Raji, Cherry, and Lamont EBV isolates have similar U2 domains and encode similar-size EBNA2 proteins, while AG876, Jijoye, and P3HR-1 have variant or absent U2 domains and variant or absent EBNA2 proteins. The AG876 U2 open reading frame and EBNA2 protein are both shorter than those of B95-8. These data indicate that the U2 open reading frame encodes EBNA2.
对B95 - 8和AG876这两种爱泼斯坦 - 巴尔病毒（EBV）分离株的U2区域进行序列分析，结果显示，在一个先前确定的长开放阅读框内存在差异，该开放阅读框编码潜伏感染、生长转化的B淋巴细胞系中表达的一种病毒RNA 3'端1.5千碱基的序列。U2开放阅读框内EBV分离株之间的差异与一种EBV核抗原EBNA2的差异相关。B95 - 8、W91、Raji、Cherry和Lamont EBV分离株具有相似的U2结构域，并编码大小相似的EBNA2蛋白，而AG876、Jijoye和P3HR - 1具有变异的或缺失的U2结构域以及变异的或缺失的EBNA2蛋白。AG876的U2开放阅读框和EBNA2蛋白均比B95 - 8的短。这些数据表明，U2开放阅读框编码EBNA2。
