Deininger P L
Anal Biochem. 1983 Feb 15;129(1):216-23. doi: 10.1016/0003-2697(83)90072-6.
A method for producing random subclones using sonication to fragment the DNA is presented. The sonication is combined with enzymatic repair of the fragment ends and a rigorous size fractionation step to prepare subclones of relatively homogeneous and specific size. Under some conditions sonication is shown to shear A + T-rich sequences preferentially, although under most conditions it will create a random subclone library. The use of these subclone libraries for an improved "shotgun" DNA sequencing strategy is tested on a 17.2-kb (kilobase) fragment of Epstein-Barr virus.
本文介绍了一种利用超声破碎DNA来产生随机亚克隆的方法。超声处理与片段末端的酶促修复以及严格的大小分级步骤相结合，以制备具有相对均一和特定大小的亚克隆。在某些条件下，超声处理显示优先剪切富含A+T的序列，尽管在大多数情况下它会创建一个随机亚克隆文库。这些亚克隆文库在改进的“鸟枪法”DNA测序策略中的应用，在爱泼斯坦-巴尔病毒的一个17.2千碱基（kb）片段上进行了测试。
