Blombäck M, Chmielewska J, Netré C, Akerblom O
Vox Sang. 1984;47(5):335-42. doi: 10.1111/j.1423-0410.1984.tb04136.x.
This study deals with the question of how blood coagulation, kallikrein and fibrinolytic systems are affected by storage of plasma at +6 degrees C. Blood was collected into citrate phosphate dextrose adenine (CPD) or acid citrate dextrose (ACD) and the plasma samples were stored at +6 degrees C for 35 days. Samples were taken at weekly intervals for assays of various parameters of the different systems. No significant changes were observed in the levels of the main thrombin inhibitor, antithrombin III. At the end of the storage period, however, fibrinopeptide A levels increased markedly, particularly in the ACD plasma, indicating thrombin activation. There was no change in the plasminogen level, but a decrease in the levels of antiplasmin and urokinase inhibitors and an increase in the level of the fibrinogen degradation fragment B beta 15-42 were observed, indicating activation of the fibrinolytic system. The level of antikallikrein activity decreased sharply in ACD plasma; CPD plasma was less affected. This decrease was parallel to the increase in spontaneous proteolytic activity and correlated with the increase in fibrinopeptide A. Prolonged storage of plasma of +6 degrees C thus resulted in the activation of coagulation, fibrinolytic and kallikrein systems and decrease in inhibitors. The activation was much more pronounced in ACD than in CPD plasma.
本研究探讨了血浆在6℃储存时凝血、激肽释放酶和纤溶系统如何受到影响。血液采集到枸橼酸盐磷酸盐葡萄糖腺嘌呤（CPD）或酸性枸橼酸盐葡萄糖（ACD）中，血浆样本在6℃储存35天。每周采集样本，检测不同系统的各种参数。主要凝血酶抑制剂抗凝血酶III的水平未观察到显著变化。然而，在储存期结束时，纤维蛋白肽A水平显著升高，特别是在ACD血浆中，表明凝血酶激活。纤溶酶原水平没有变化，但抗纤溶酶和尿激酶抑制剂水平降低，纤维蛋白原降解片段Bβ15 - 42水平升高，表明纤溶系统激活。ACD血浆中抗激肽释放酶活性水平急剧下降；CPD血浆受影响较小。这种下降与自发蛋白水解活性的增加平行，并与纤维蛋白肽A的增加相关。因此，血浆在6℃长时间储存导致凝血、纤溶和激肽释放酶系统激活以及抑制剂减少。ACD血浆中的激活比CPD血浆中更明显。
