Fiedler H P, Wachter J
Universität Tübingen, Biologisches Institut, LB Mikrobiologie/Antibiotika, F.R.G.
J Chromatogr. 1991 Jan 4;536(1-2):343-7. doi: 10.1016/s0021-9673(01)89268-2.
A rapid and specific ion-pair reversed-phase high-performance liquid chromatographic method was developed for the determination of bleomycins. The use of 5-microns particles of less adsorptive reversed-phase packings and sodium perchlorate as ion-pairing reagent permitted a short analysis time and the transferability of the separations on different batches of the reversed-phase materials. The detection sensitivity and precision of the method demonstrated that the system is suitable for routine analysis.
建立了一种快速、特异的离子对反相高效液相色谱法测定博来霉素。使用5微米的低吸附反相填料颗粒和高氯酸钠作为离子对试剂，可缩短分析时间，并能在不同批次的反相材料上实现分离的可转移性。该方法的检测灵敏度和精密度表明该系统适用于常规分析。
