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1
Protease La from Escherichia coli hydrolyzes ATP and proteins in a linked fashion.来自大肠杆菌的蛋白酶La以一种连锁方式水解ATP和蛋白质。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4883-7. doi: 10.1073/pnas.79.16.4883.
2
The role of ATP hydrolysis in the breakdown of proteins and peptides by protease La from Escherichia coli.ATP水解在大肠杆菌蛋白酶La分解蛋白质和肽过程中的作用。
J Biol Chem. 1985 Oct 5;260(22):12029-34.
3
Selectivity of intracellular proteolysis: protein substrates activate the ATP-dependent protease (La).细胞内蛋白水解的选择性：蛋白质底物激活ATP依赖性蛋白酶（La）。
Science. 1986 Apr 25;232(4749):500-3. doi: 10.1126/science.2938257.
4
Protease La, the lon gene product, cleaves specific fluorogenic peptides in an ATP-dependent reaction.蛋白酶La，即lon基因产物，在依赖ATP的反应中切割特定的荧光肽。
J Biol Chem. 1985 Oct 5;260(22):12022-8.
5
Escherichia coli contains a soluble ATP-dependent protease (Ti) distinct from protease La.大肠杆菌含有一种与蛋白酶La不同的可溶性ATP依赖性蛋白酶（Ti）。
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5550-4. doi: 10.1073/pnas.84.16.5550.
6
ATP hydrolysis-dependent protease activity of the lon (capR) protein of Escherichia coli K-12.大肠杆菌K-12的lon（capR）蛋白的ATP水解依赖性蛋白酶活性。
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4728-32. doi: 10.1073/pnas.78.8.4728.
7
The energy utilized in protein breakdown by the ATP-dependent protease (La) from Escherichia coli.大肠杆菌中依赖ATP的蛋白酶（La）在蛋白质分解过程中所利用的能量。
J Biol Chem. 1987 Jan 15;262(2):722-6.
8
The heat-shock protein HslVU from Escherichia coli is a protein-activated ATPase as well as an ATP-dependent proteinase.来自大肠杆菌的热休克蛋白HslVU是一种蛋白激活的ATP酶，也是一种ATP依赖性蛋白酶。
Eur J Biochem. 1997 Aug 1;247(3):1143-50. doi: 10.1111/j.1432-1033.1997.01143.x.
9
DNA stimulates ATP-dependent proteolysis and protein-dependent ATPase activity of protease La from Escherichia coli.DNA刺激来自大肠杆菌的蛋白酶La的ATP依赖性蛋白水解作用和蛋白依赖性ATP酶活性。
Proc Natl Acad Sci U S A. 1982 Feb;79(3):795-9. doi: 10.1073/pnas.79.3.795.
10
ATP hydrolysis is not stoichiometrically linked with proteolysis in the ATP-dependent protease La from Escherichia coli.在来自大肠杆菌的ATP依赖性蛋白酶La中，ATP水解与蛋白水解在化学计量上没有关联。
J Biol Chem. 1993 Oct 25;268(30):22502-7.

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Roles of LonP1 in Oral-Maxillofacial Developmental Defects and Tumors: A Novel Insight.LonP1 在口腔颌面发育缺陷和肿瘤中的作用：新的见解。
Int J Mol Sci. 2022 Nov 2;23(21):13370. doi: 10.3390/ijms232113370.
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Leveraging Peptide Substrate Libraries to Design Inhibitors of Bacterial Lon Protease.利用肽底物文库设计细菌 Lon 蛋白酶抑制剂。
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Proteolysis mediated by the membrane-integrated ATP-dependent protease FtsH has a unique nonlinear dependence on ATP hydrolysis rates.膜整合 ATP 依赖型蛋白酶 FtsH 介导的蛋白水解作用对 ATP 水解速率具有独特的非线性依赖性。
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The Role of Proteases in the Virulence of Plant Pathogenic Bacteria.蛋白酶在植物病原菌毒力中的作用。
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Regulated Proteolysis in Bacteria: Caulobacter.细菌中的调控性蛋白水解作用：柄杆菌属
Annu Rev Genet. 2016 Nov 23;50:423-445. doi: 10.1146/annurev-genet-120215-035235. Epub 2016 Oct 13.
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The active ClpP protease from M. tuberculosis is a complex composed of a heptameric ClpP1 and a ClpP2 ring.结核分枝杆菌的活性 ClpP 蛋白酶是一种由七聚体 ClpP1 和 ClpP2 环组成的复合物。
EMBO J. 2012 Mar 21;31(6):1529-41. doi: 10.1038/emboj.2012.5. Epub 2012 Jan 27.
7
Multitasking in the mitochondrion by the ATP-dependent Lon protease.线粒体中由ATP依赖型Lon蛋白酶执行的多任务处理。
Biochim Biophys Acta. 2012 Jan;1823(1):56-66. doi: 10.1016/j.bbamcr.2011.11.003. Epub 2011 Nov 18.
8
Binding and cleavage of E. coli HUbeta by the E. coli Lon protease.大肠杆菌 Lon 蛋白酶对 E. coli HUβ 的结合与切割。
Biophys J. 2010 Jan 6;98(1):129-37. doi: 10.1016/j.bpj.2009.09.052.
9
Functional mechanics of the ATP-dependent Lon protease- lessons from endogenous protein and synthetic peptide substrates.ATP 依赖性 Lon 蛋白酶的功能机制——来自内源性蛋白质和合成肽底物的经验教训
Biochim Biophys Acta. 2008 May;1784(5):727-35. doi: 10.1016/j.bbapap.2008.02.010. Epub 2008 Mar 5.
10
Slicing a protease: structural features of the ATP-dependent Lon proteases gleaned from investigations of isolated domains.剖析一种蛋白酶：从对分离结构域的研究中获得的ATP依赖型Lon蛋白酶的结构特征
Protein Sci. 2006 Aug;15(8):1815-28. doi: 10.1110/ps.052069306.

本文引用的文献

1
Proteolytic enzymes.蛋白水解酶
Annu Rev Biochem. 1960;29:45-72. doi: 10.1146/annurev.bi.29.070160.000401.
2
Federation of American Societies for Experimental Biology. 66th Annual meeting. New Orleans, Louisiana, April 15-23, 1982. Abstracts of papers 3478-6993.美国实验生物学学会联合会。第66届年会。路易斯安那州新奥尔良，1982年4月15日至23日。论文摘要3478 - 6993
Fed Proc. 1982 Mar 5;41(4):865-1466.
3
Liver mitochondria contain an ATP-dependent, vanadate-sensitive pathway for the degradation of proteins.肝脏线粒体含有一条依赖ATP且对钒酸盐敏感的蛋白质降解途径。
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1869-73. doi: 10.1073/pnas.79.6.1869.
4
Proteases in Escherichia coli.大肠杆菌中的蛋白酶
Methods Enzymol. 1981;80 Pt C:680-702. doi: 10.1016/s0076-6879(81)80052-3.
5
Studies of the ATP-dependent proteolytic enzyme, protease La, from Escherichia coli.对来自大肠杆菌的ATP依赖性蛋白水解酶蛋白酶La的研究。
J Biol Chem. 1982 Apr 25;257(8):4187-95.
6
E. coli contains eight soluble proteolytic activities, one being ATP dependent.大肠杆菌含有八种可溶性蛋白水解活性，其中一种依赖于ATP。
Nature. 1981 Aug 13;292(5824):652-4. doi: 10.1038/292652a0.
7
ATP-stimulated endoprotease is associated with the cell membrane of E. coli.ATP刺激的内蛋白酶与大肠杆菌的细胞膜相关联。
Nature. 1981 Apr 2;290(5805):419-21. doi: 10.1038/290419a0.
8
Control of proteolysis.蛋白水解的控制
Annu Rev Biochem. 1980;49:63-91. doi: 10.1146/annurev.bi.49.070180.000431.
9
Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis.ATP在蛋白质分解中的推测作用：蛋白质与ATP依赖性蛋白水解多肽的多条链结合。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1783-6. doi: 10.1073/pnas.77.4.1783.
10
ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation.网织红细胞蛋白与蛋白质降解所需多肽的ATP依赖性缀合。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1365-8. doi: 10.1073/pnas.77.3.1365.

来自大肠杆菌的蛋白酶La以一种连锁方式水解ATP和蛋白质。

Protease La from Escherichia coli hydrolyzes ATP and proteins in a linked fashion.

作者信息

Waxman L, Goldberg A L

出版信息

Proc Natl Acad Sci U S A. 1982 Aug;79(16):4883-7. doi: 10.1073/pnas.79.16.4883.

DOI:10.1073/pnas.79.16.4883
PMID:6214787
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346789/
Abstract

The energy requirement for protein breakdown in Escherichia coli results from an ATP requirement for the function of protease La, the product of the lon gene. This novel serine protease contains an ATPase activity that is essential for proteolysis. ATP and protein hydrolysis show the same Km for ATP (30-40 muM) and are affected similarly by various inhibitors, activators, and ATP analogs. Vanadate inhibited ATP cleavage and caused a proportionate reduction in casein hydrolysis, and inhibitors of serine proteases reduced ATP cleavage. Thus, ATP and protein hydrolysis appear to be linked stoichiometrically. Furthermore, ATP hydrolysis is stimulated two- to threefold by polypeptides that are substrates for the protease (casein, glucagon) but not by nonhydrolyzed polypeptides (insulin, RNase). Unlike hemoglobin or native albumin, globin and denatured albumin stimulated ATP hydrolysis and were substrates for proteolysis. It is suggested that the stimulation of ATP hydrolysis by potential substrates triggers activation of the proteolytic function.

摘要

大肠杆菌中蛋白质分解的能量需求源于蛋白酶La（lon基因的产物）功能对ATP的需求。这种新型丝氨酸蛋白酶含有一种对蛋白水解至关重要的ATP酶活性。ATP和蛋白质水解对ATP具有相同的Km值（30 - 40μM），并且受到各种抑制剂、激活剂和ATP类似物的影响相似。钒酸盐抑制ATP裂解，并导致酪蛋白水解成比例减少，丝氨酸蛋白酶抑制剂减少ATP裂解。因此，ATP和蛋白质水解似乎在化学计量上是相关的。此外，蛋白酶的底物多肽（酪蛋白、胰高血糖素）可将ATP水解刺激两到三倍，但非水解多肽（胰岛素、核糖核酸酶）则不能。与血红蛋白或天然白蛋白不同，球蛋白和变性白蛋白刺激ATP水解并且是蛋白水解的底物。有人提出，潜在底物对ATP水解的刺激触发了蛋白水解功能的激活。