Knapp W, Berger R, Posch B
Immunopharmacology. 1982 Oct;5(1):1-9. doi: 10.1016/0162-3109(82)90031-5.
Hydrocortisone (HC), when added in pharmacologically attainable concentrations (10(-6)M) to pokeweed mitogen (PWM) cultures of unseparated human mononuclear cells (MNC) caused a marked enhancement of the in vitro secretion of all three major immunoglobulin (Ig) classes and of two out of three antibody specificities tested x (Ig) M anti-IgG antibodies and anti ss-DNA antibodies, but not anti-tetanus toxoid antibodies). Such an effect was not observed when HC was added to cocultures of isolated B cells with mitomycin C-treated autologous T cells. This result suggests, that HC effects on T-mediated suppressor mechanisms--rather than direct stimulatory effects--on B cells are involved in the observed enhancement of PWM induced in vitro B cell differentiation. One possible explanation for this phenomenon might be that HC, by inhibiting interleukin 2 release, inhibits also suppressor T-cell acitivation. This hypothesis would also fit the observation that HC significantly inhibits concanavalin A-mediated suppression of PWM-induced B cell differentiation, but that it has no effect on in vivo preactivated suppressor T cells from human cord blood.
当以药理学可达到的浓度（10⁻⁶M）将氢化可的松（HC）添加到未分离的人单核细胞（MNC）的商陆丝裂原（PWM）培养物中时，会导致所有三种主要免疫球蛋白（Ig）类别的体外分泌以及所测试的三种抗体特异性中的两种（IgM抗IgG抗体和抗单链DNA抗体，但不包括抗破伤风类毒素抗体）显著增强。当将HC添加到经丝裂霉素C处理的自体T细胞与分离的B细胞的共培养物中时，未观察到这种效应。该结果表明，HC对T细胞介导的抑制机制的作用——而非对B细胞的直接刺激作用——参与了所观察到的PWM诱导的体外B细胞分化增强。对此现象的一种可能解释可能是，HC通过抑制白细胞介素2的释放，也抑制了抑制性T细胞的激活。这一假设也符合以下观察结果：HC显著抑制伴刀豆球蛋白A介导的对PWM诱导的B细胞分化的抑制作用，但对来自人脐血的体内预激活抑制性T细胞没有影响。
