Venkataramanan R, Axelson J E
J Pharm Sci. 1978 Feb;67(2):201-5. doi: 10.1002/jps.2600670219.
A sensitive and specific electron-capture detector GLC method capable of detecting picogram quantities of tocainide, a lidocaine analog, in biological fluids was developed. This method consists of extracting the compound into methylene chloride and derivatizing with heptafluorobutyric anhydride to form the monoheptafluorobutyryl derivative. The derivative formation was confirmed by GLC-mass spectrometry. Quantitative estimation was performed using 1-bromonaphthalene as an internal standard. The minimum detectable level by the electron-capture method was approximately 30 pg/injection as opposed to approximately 3 ng/injection with a flame-ionization detector Linear response was observed in the range from 50 pg to 3 ng using an electron-capture detector. No interference from endogenous substances was observed.
已开发出一种灵敏且特异的电子捕获检测器气相色谱法，该方法能够检测生物流体中皮克量的妥卡尼（一种利多卡因类似物）。此方法包括将化合物萃取到二氯甲烷中，并用七氟丁酸酐进行衍生化反应以形成单七氟丁酰衍生物。通过气相色谱 - 质谱联用仪确认了衍生物的形成。使用1 - 溴萘作为内标进行定量测定。电子捕获法的最低检测限约为30皮克/进样，而火焰离子化检测器的最低检测限约为3纳克/进样。使用电子捕获检测器时，在50皮克至3纳克的范围内观察到线性响应。未观察到内源性物质的干扰。
