用气相色谱法测定保泰松γ-氧代代谢物的血浆浓度。

GLC determination of plasma concentrations of gamma-oxo metabolite of phenylbutazone.

作者信息

Midha K K, McGilveray I J, Cooper J K

出版信息

J Pharm Sci. 1978 Feb;67(2):279-81. doi: 10.1002/jps.2600670246.

Abstract

A quantitative method for the gamma-oxo metabolite of phenylbutazone from plasma is described. The procedure involved an ethylene dichloride extraction of acidified plasma to which an internal standard, acenocoumarol, had been added. The extracted gamma-oxo metabolite and the internal standard were methylated and analyzed by GLC. Determination of 0.25 microgram of gamma-oxo metabolite/ml with a relative standard deviation of 6.5% was accomplished.

摘要

本文描述了一种从血浆中定量分析保泰松γ-氧代代谢物的方法。该方法包括用二氯乙烷萃取酸化血浆，其中已添加内标醋硝香豆素。萃取得到的γ-氧代代谢物和内标进行甲基化，然后用气相色谱法分析。该方法可测定血浆中浓度为0.25微克/毫升的γ-氧代代谢物，相对标准偏差为6.5%。