Podesta M, Frassoni F, Van Lint M T, Piaggio G, Giordano D, Cerri R, Marmont A, Bacigalupo A
Thymus. 1982 Jul;4(4):233-42.
Bone marrow and peripheral blood T cells were obtained from 15 normal individuals by E rosetting and cultured in round-bottomed microwells for 7 days in RPMI or in RPMI supplemented with mitogens (pokeweed mitogen, phytohemagglutinin or concanavalin A). Supernatants and cells were harvested on days 1, 2, 3, 4 and 7 and co-cultured with normal marrow cells in semi-solid agar to test their CFU-c suppressor activity. The results of this study indicate that (a) RPMI treated cells and their supernatants have no effect or an enhancing effect on CFU-c growth; (b) all 3 mitogens generate CFU-c suppressor T cells on day 1 of culture; (c) the inhibitory activity is detectable until day 4 of culture, though overall reduced, and is completely lost on day; 7 (d) the trend for supernatants of mitogen-treated T cells is quite similar with a tendency to complete loss of the inhibitory effect on day 7. We interpret these data as indicating that T cells release a soluble inhibitor of CFU-c growth within a few hours from polyclonal activation, the production of which is either controlled or lost with time in culture.
通过E花环法从15名正常个体获取骨髓和外周血T细胞，并在圆底微孔板中于RPMI培养基或添加有丝分裂原（商陆有丝分裂原、植物血凝素或刀豆球蛋白A）的RPMI培养基中培养7天。在第1、2、3、4和7天收获上清液和细胞，并与正常骨髓细胞在半固体琼脂中共培养，以测试它们对集落形成单位 - 集落（CFU - c）的抑制活性。本研究结果表明：（a）RPMI处理的细胞及其上清液对CFU - c生长无影响或有增强作用；（b）所有3种有丝分裂原在培养第1天均可产生CFU - c抑制性T细胞；（c）抑制活性在培养第4天之前均可检测到，尽管总体上有所降低，而在第7天完全丧失；（d）有丝分裂原处理的T细胞上清液的趋势非常相似，在第7天有抑制作用完全丧失的趋势。我们将这些数据解释为表明T细胞在多克隆激活后的数小时内释放一种CFU - c生长的可溶性抑制剂，其产生在培养过程中随时间受到控制或丧失。
