Bacigalupo A, Mingari M C, Melioli G, Frassoni F, Podestá M, Moretta L, Van Lint M T, Marmont A M
Exp Hematol. 1983 Jul;11(6):506-11.
T derived colony inhibitory activity (Td/CIA) was obtained from unstimulated T cells from aplastic anemia patients (SAA), or from PWM primed normal T cells. Td/CIA suppressed CFUC growth of normal allogeneic marrow to less than 30% of expected growth. Td/CIA was then added to normal peripheral blood T and B cells, primed with PWM, to test whether it would interfere with in vitro immunoglobulin (Ig) production. When Td/CIA from normal T cells was added to cultures of T + B cells + PWM there was a 2-2.1-fold increase in Ig production. Similarly the addition of Td/CIA from SAA patients also resulted in a 1.4 up to 166-fold increase in Ig production. These results indicate that either (a) the targets for Td/CIA are expressed on hemopoietic but not on T and B cells, or (b) that Td/CIA inactivates an accessory cell which is essential for CFUC growth but not for the PWM driven in vitro B cell differentiation system.
T衍生集落抑制活性（Td/CIA）来自再生障碍性贫血患者（重型再生障碍性贫血）未受刺激的T细胞，或来自经美洲商陆有丝分裂原（PWM）致敏的正常T细胞。Td/CIA将正常同种异体骨髓的集落形成单位（CFUC）生长抑制至预期生长的30%以下。然后将Td/CIA添加到经PWM致敏的正常外周血T细胞和B细胞中，以测试它是否会干扰体外免疫球蛋白（Ig）的产生。当将来自正常T细胞的Td/CIA添加到T+B细胞+PWM的培养物中时，Ig产量增加了2至2.1倍。同样，添加来自再生障碍性贫血患者的Td/CIA也导致Ig产量增加了1.4至166倍。这些结果表明，要么（a）Td/CIA的靶标在造血细胞上表达，而不在T细胞和B细胞上表达，要么（b）Td/CIA使一种辅助细胞失活，该辅助细胞对CFUC生长至关重要，但对PWM驱动的体外B细胞分化系统不重要。
