Perlman J, Feldman J F
Mol Cell Biol. 1982 Oct;2(10):1167-73. doi: 10.1128/mcb.2.10.1167-1173.1982.
Treatment of Neurospora crassa with 0.1 microgram of cycloheximide per ml, a concentration which inhibited protein synthesis by about 70%, resulted in the greatly enhanced synthesis of at least three polypeptide bands with estimated molecular weights of 88,000, 30,000, and 28,000. A temperature shift from 25 to 37 degrees C resulted in the appearance of a single new polypeptide band of 70,000 daltons, the same size as the major heat shock-induced proteins observed in species of Drosophila and Dictyostelium. Synthesis of the cycloheximide-stimulated polypeptide bands was on cytoplasmic ribosomes rather than on mitochondrial ribosomes, as incorporation of isotope into the polypeptide bands was inhibited by 1.0 microgram of cycloheximide per ml but not by 1 mg of chloramphenicol per ml. In a mutant with cycloheximide-resistant ribosomes, 0.1 microgram of cycloheximide per ml failed to alter the pattern of protein synthesis from that of the controls. It is suggested that the new synthesis of the polypeptide bands reflects specific mechanisms of adaptation to different kinds of environmental stress, including inhibition of protein synthesis and temperature increases.
用每毫升含0.1微克放线菌酮处理粗糙脉孢菌，该浓度能抑制约70%的蛋白质合成，结果导致至少三条多肽带的合成显著增强，其估计分子量分别为88,000、30,000和28,000。温度从25摄氏度升至37摄氏度会导致出现一条分子量为70,000道尔顿的新多肽带，其大小与在果蝇和盘基网柄菌中观察到的主要热休克诱导蛋白相同。放线菌酮刺激的多肽带的合成发生在细胞质核糖体上而非线粒体核糖体上，因为每毫升1.0微克放线菌酮会抑制同位素掺入多肽带，但每毫升1毫克氯霉素则不会。在具有抗放线菌酮核糖体的突变体中，每毫升0.1微克放线菌酮未能改变蛋白质合成模式，使其与对照不同。有人提出，多肽带的新合成反映了对不同类型环境压力的特定适应机制，包括蛋白质合成抑制和温度升高。
