Petersen N S, Mitchell H K
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1708-11. doi: 10.1073/pnas.78.3.1708.
A mild heat shock at 35 degrees C, which induces heat shock gene expression, greatly enhances survival and the recovery of protein synthesis in Drosophila cells after a higher temperature heat shock. The 35 degrees C treatment is also effective in preventing heat-induced developmental defects in pupae. We show here that the major larval mRNAs are present in approximately normal (25 degrees C) concentrations after a 40.1 degrees C heat shock whether or not the animals receive a pretreatment. This indicates that the pretreatment affects translation directly rather than messenger concentration. We also observe selective translation of heat shock messages and some 25 degrees C messages during recovery from heat shock.
在35摄氏度下进行的轻度热休克可诱导热休克基因表达，极大地提高果蝇细胞在更高温度热休克后的存活率和蛋白质合成的恢复能力。35摄氏度的处理在预防蛹期热诱导的发育缺陷方面也很有效。我们在此表明，无论动物是否接受预处理，在40.1摄氏度热休克后，主要的幼虫mRNA浓度大致处于正常（25摄氏度）水平。这表明预处理直接影响翻译而非信使浓度。我们还观察到在热休克恢复过程中热休克信息和一些25摄氏度信息的选择性翻译。
