Smith S S, Ashburn F S, Pilkerton A R, Recant L
Retina. 1982;2(2):121-5. doi: 10.1097/00006982-198200220-00009.
Fluorophotometry was performed on rats eight to ten days after they were made diabetic with streptozotocin (85 mg/kg), alloxan (50 mg/kg), or pancreatectomy. When compared with normal control animals, no significant differences in aqueous or vitreous fluorescein levels were noted in any of the diabetic models studied. We did note significant depressions of plasma fluorescein levels in pancreatectomized and streptozotocin-treated rats. We normalized our data for these plasma differences by calculating the ratios of aqueous and vitreous fluorescein to plasma fluorescein levels (RA and RV). Both RA and RV were significantly elevated in streptozotocin-induced diabetes; RV alone was elevated following pancreatectomy, while no change in either ratio was apparent in diabetes induced with alloxan. Treatment of streptozotocin-diabetic rats with cortisol for one week caused plasma fluorescein levels and RV to return toward normal, although RA remained elevated. We are cautious in assigning any meaning to changes in RA and RV, especially in view of the paucity of information on the behavior of fluorescein in the blood.
在用链脲佐菌素（85毫克/千克）、四氧嘧啶（50毫克/千克）使大鼠患糖尿病或进行胰腺切除术后八至十天，对大鼠进行了荧光光度测定。与正常对照动物相比，在所研究的任何糖尿病模型中，房水或玻璃体液中的荧光素水平均未发现显著差异。我们确实注意到，胰腺切除和链脲佐菌素处理的大鼠血浆荧光素水平显著降低。我们通过计算房水和玻璃体液中荧光素与血浆荧光素水平的比值（RA和RV）对这些血浆差异的数据进行了标准化处理。在链脲佐菌素诱导的糖尿病中，RA和RV均显著升高；胰腺切除术后仅RV升高，而在四氧嘧啶诱导的糖尿病中，两种比值均无明显变化。用皮质醇治疗链脲佐菌素诱导的糖尿病大鼠一周，可使血浆荧光素水平和RV恢复正常，尽管RA仍升高。我们对赋予RA和RV变化任何意义持谨慎态度，特别是考虑到关于荧光素在血液中行为的信息匮乏。
