Blair N P, Jones C W, Rusin M M
Arch Ophthalmol. 1984 Dec;102(12):1810-4. doi: 10.1001/archopht.1984.01040031468025.
Carboxyfluorescein resembles fluorescein in size and spectral characteristics but is much less lipid soluble. Both dyes were used to differentiate between two groups of factors that influence penetration across the blood-retinal barrier: (1) factors that depend on lipid solubility, such as the area of the barrier, and (2) factors independent of lipid solubility, such as opened intercellular junctions or necrotic cells. Vitreous fluorophotometry was performed on normal and diabetic rats after injection of either dye. After the results were adjusted for sources of error, midvitreous-plasma dye ratios for carboxyfluorescein and fluorescein were of the same order of magnitude in normal rats. Ratios for both dyes increased in diabetic rats, and the increases were similar in magnitude. Our results suggest that lipid solubility contributes little to inward transport of these dyes in both the normal and diabetic states.
羧基荧光素在大小和光谱特性上与荧光素相似，但脂溶性要低得多。这两种染料被用于区分影响穿过血视网膜屏障的两组因素：（1）取决于脂溶性的因素，如屏障面积；（2）与脂溶性无关的因素，如开放的细胞间连接或坏死细胞。在正常和糖尿病大鼠注射其中一种染料后进行玻璃体荧光光度测定。对误差来源进行校正后，正常大鼠中羧基荧光素和荧光素的玻璃体内-血浆染料比率处于同一数量级。两种染料的比率在糖尿病大鼠中均升高，且升高幅度相似。我们的结果表明，在正常和糖尿病状态下，脂溶性对这些染料的内向转运贡献很小。
