Vine A K, Kisly A M, Betz A L, Howatt W F
Arch Ophthalmol. 1984 Jul;102(7):1083-5. doi: 10.1001/archopht.1984.01040030877038.
Vitreous fluorophotometry (VF) has been used to study the blood-retinal barrier in rats with streptozocin-induced diabetes. Subsequent investigations have shown that many variables other than diabetes influence VF values. With the use of a commercially available fluorophotometer, we assessed VF measurements in diabetic rats while simultaneously measuring other variables, including plasma fluorescein level, free plasma fluorescein level, and blood pH. Mean total plasma fluorescein concentration, mean free plasma fluorescein concentration, and mean midvitreous fluorescein concentration were significantly decreased in diabetic animals. The mean ratio of midvitreous fluorescein concentration to total plasma fluorescein concentration was significantly increased in diabetic animals. In comparison with nondiabetic animals, the diabetic animals showed no significant difference in blood pH or mean ratio of free plasma fluorescein concentration to total plasma fluorescein concentration. Our findings confirm an early derangement of the blood-retinal barrier in diabetic rats.
玻璃体荧光光度测定法（VF）已被用于研究链脲佐菌素诱导的糖尿病大鼠的血视网膜屏障。随后的研究表明，除糖尿病外，许多变量都会影响VF值。我们使用市售的荧光光度计，在测量糖尿病大鼠VF值的同时，还测量了其他变量，包括血浆荧光素水平、游离血浆荧光素水平和血液pH值。糖尿病动物的平均总血浆荧光素浓度、平均游离血浆荧光素浓度和平均玻璃体中部荧光素浓度显著降低。糖尿病动物玻璃体中部荧光素浓度与总血浆荧光素浓度的平均比值显著升高。与非糖尿病动物相比，糖尿病动物的血液pH值或游离血浆荧光素浓度与总血浆荧光素浓度的平均比值无显著差异。我们的研究结果证实了糖尿病大鼠血视网膜屏障的早期紊乱。
