Rhode S L
J Virol. 1978 Jan;25(1):215-23. doi: 10.1128/JVI.25.1.215-223.1978.
A temperature-sensitive mutant of H-1, ts14, that is partially defective in replicative-form (RF) DNA synthesis has been isolated. ts14 H-1 is characterized by a decrease in plaque-forming ability and production of infectious virus at the restrictive temperature of 39.5 degrees C. RF DNA synthesis of ts14 is reduced to 3 to 7% of that of wild-type H-1 at either the restrictive or the permissive temperature. A complementation analysis of RF synthesis of ts14 and a viable defective H-1 virus, DI-1, or wild-type H-3 indicates that the defective RF DNA synthesis of ts14 is cis-acting. ts14, unlike wild-type H-1, causes a multiplicity-dependent inhibition of DI-1 or H-3, but not LuIII, RF DNA synthesis. Mixed infections of cells with two parvoviruses also exhibited a cross-interference for viral protein synthesis that was multiplicity dependent, ts14 inhibited infectious virus production of H-1 or H-3, but not LuIII. LuIII-or H-3-pseudotype particles were produced by coinfection with H-1. H-3 and H-1 showed similar interactions with ts14, and H-3 DNA was more homologous to H-1 than was LuIII by comparative physical mapping studies. The results suggest that ts14 is a mutant with a defect in a regulatory sequence of its DNA that influence RF DNA replication.
已分离出H-1的温度敏感突变体ts14，其在复制型（RF）DNA合成方面存在部分缺陷。ts14 H-1的特征是在39.5℃的限制温度下，噬斑形成能力和传染性病毒产量下降。在限制温度或允许温度下，ts14的RF DNA合成降至野生型H-1的3%至7%。对ts14与一种存活的缺陷型H-1病毒DI-1或野生型H-3的RF合成进行互补分析表明，ts14的缺陷型RF DNA合成是顺式作用的。与野生型H-1不同，ts14会导致DI-1或H-3而非LuIII的RF DNA合成出现多倍体依赖性抑制。两种细小病毒对细胞的混合感染也表现出对病毒蛋白合成的交叉干扰，这种干扰是多倍体依赖性的，ts14抑制H-1或H-3而非LuIII的传染性病毒产生。通过与H-1共感染产生了LuIII或H-3假型颗粒。H-3和H-1与ts14表现出相似的相互作用，并且通过比较物理图谱研究表明，H-3 DNA与H-1的同源性比与LuIII的更高。结果表明，ts14是一种其DNA调控序列存在缺陷的突变体，该缺陷影响RF DNA复制。
