通过限制性内切酶消化产物的比较来估计DNA序列差异。
Estimation of DNA sequence divergence from comparison of restriction endonuclease digests.
作者信息
Upholt W B
出版信息
Nucleic Acids Res. 1977;4(5):1257-65. doi: 10.1093/nar/4.5.1257.
Abstract
An estimation of the DNA sequence divergence between defined DNA secquences of individuals or species may be made from comparison by gel electrophoresis of restriction endonuclease digests. This analysis is applicable to purified DNA sequence of moderate complexity (1-100 X 10(6) daltons) which have diverged by base substitution of 0.5 to 25% of nucleotides.
摘要
通过对限制内切酶消化产物进行凝胶电泳比较，可以估计个体或物种特定DNA序列之间的DNA序列差异。这种分析适用于中等复杂度（1-100×10⁶道尔顿）的纯化DNA序列，这些序列因碱基替换而发生了0.5%至25%的核苷酸差异。
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