Cabrera C V, Esteban M
J Virol. 1978 Jan;25(1):442-5. doi: 10.1128/JVI.25.1.442-445.1978.
A procedure for the isolation of intact vaccinia DNA molecules by chromatography on hydroxyapatite in the presence of 6 M urea is described. When lysates of virions containing 0.5 to 10 microgram of DNA were employed, over 95% of the viral DNA could be recovered free of poteins. Vaccinia DNA molecules isolated in this manner sedimented at 68S in neutral sucrose gradients and had an average contour length of 62.3 micrometer when examined in an electron microscope, and the DNA could be cleaved with the restriction endonuclease EcoRI and BamHI. The results of these analyses showed that intact vaccinia DNA molecules of 120 X 10(6) to 130 X 10(6) molecular weight could be obtained by the procedures described.
本文描述了一种在6M尿素存在下通过羟基磷灰石柱色谱法分离完整痘苗病毒DNA分子的方法。当使用含有0.5至10微克DNA的病毒粒子裂解物时，超过95%的病毒DNA可以在无蛋白质的情况下回收。以这种方式分离的痘苗病毒DNA分子在中性蔗糖梯度中沉降系数为68S，在电子显微镜下检查时平均轮廓长度为62.3微米，并且该DNA可以被限制性内切酶EcoRI和BamHI切割。这些分析结果表明，通过所述方法可以获得分子量为120×10⁶至130×10⁶的完整痘苗病毒DNA分子。
