Simonian S Z, Antonova T P, Chernos V I, Nosikov V V
Mol Biol (Mosk). 1979 Nov-Dec;13(6):1255-61.
Restriction endonucleases EcoRI, BamHI, Hind III and KpnI were used for analysis of acccinia virus DNA. The number and size of restriction endonuclease fragments were determined by gel electrophoresis and the analysis of 3H-labeled vaccinia virus DNA. It was shown that many EcoRI and BamHI fragments had the same and similar sizes. The exact number of EcoRI and BamHI fragments were estimated only after analysis of [3H]-labeled vaccinia DNA. The vaccinia genome sizes calculated from HindIII, KpnI and EcoRI are very close to the actual genome weight. But the sum of BamHI fragments is much lower than those determined by electron microscope method.
限制性内切酶EcoRI、BamHI、Hind III和KpnI被用于分析痘苗病毒DNA。通过凝胶电泳和对3H标记的痘苗病毒DNA进行分析，确定了限制性内切酶片段的数量和大小。结果表明，许多EcoRI和BamHI片段具有相同或相似的大小。只有在分析了[3H]标记的痘苗DNA后，才估算出EcoRI和BamHI片段的确切数量。根据HindIII、KpnI和EcoRI计算出的痘苗基因组大小与实际基因组重量非常接近。但BamHI片段的总和远低于通过电子显微镜法测定的结果。
