Pazderka F, Angeles A, Kovithavongs T, Dossetor J B
Cell Immunol. 1983 Jan;75(1):122-33. doi: 10.1016/0008-8749(83)90311-8.
T cells stimulated for 6-7 days in autologous mixed lymphocyte culture (AMLC) showed suppressive effects when added to fresh mixed cultures where autologous lymphocytes (A) were stimulated by Mitomycin C-treated allogeneic lymphocytes (Xm), in a ratio of A:Xm:AMLC-activated cells of 1:1:0.5. Both cytotoxic and proliferative activities in second cultures, as assayed after 6 days of incubation, were significantly inhibited (percentage suppression of cytotoxic activity observed in 17 experiments was 75.3 +/- 22.4; percentage suppression of proliferation was 60.6 +/- 18.2). Suppressor cells (SC) generated in AMLC were Mitomycin C sensitive and nonspecific in their action; not only A/Xm but also X/Am and X/Ym cultures were suppressed to the same extent. AMLC-Activated cells showed a considerable degree of proliferation in response to alloantigens but failed to express any cytotoxic activity against autologous or allogeneic phytohemagglutinin blasts. Thus, the inhibitory effect observed in this system is not due to cytotoxic elimination of responding or stimulating cells in the second culture but rather reflects a true regulatory (suppressive) mechanism.
在自体混合淋巴细胞培养（AMLC）中刺激6 - 7天的T细胞，当以A:Xm:AMLC激活细胞为1:1:0.5的比例添加到新鲜混合培养物中时，显示出抑制作用。在新鲜混合培养物中，自体淋巴细胞（A）由丝裂霉素C处理的同种异体淋巴细胞（Xm）刺激。孵育6天后测定，二次培养物中的细胞毒性和增殖活性均受到显著抑制（在17次实验中观察到的细胞毒性活性抑制百分比为75.3±22.4；增殖抑制百分比为60.6±18.2）。在AMLC中产生的抑制细胞（SC）对丝裂霉素C敏感，其作用是非特异性的；不仅A/Xm培养物，而且X/Am和X/Ym培养物也受到同等程度的抑制。AMLC激活的细胞对同种异体抗原表现出相当程度的增殖，但对自体或同种异体植物血凝素母细胞未表现出任何细胞毒性活性。因此，在该系统中观察到的抑制作用不是由于二次培养中反应性或刺激性细胞的细胞毒性消除，而是反映了一种真正的调节（抑制）机制。
