Lomnitzer R, Rabson A R
Immunology. 1982 Jan;45(1):7-13.
Pretreatment of normal human peripheral blood mononuclear cells (MN) with sodium periodate (NaIO4) resulted in the induction of suppressor cells. The mitogenic response of fresh allogeneic and autologous cells to phytohaemagglutinin (PHA), Concanavalin A (Con A), pokeweed mitogen (PWM), various antigens and in mixed lymphocyte culture was suppressed when NaIO4 pretreated cells were present. PWM-induced plaque-forming-cell responses were also suppressed by NaIO4-pretreated cells. Treatment of cells with mitomycin C before the NaIO4 treatment abolished the suppressive activity. A ratio of 1 : combination that resulted in the strongest suppression. Supernatants from NaIO4-pretreated cell cultures were not inhibitory.
用高碘酸钠（NaIO4）预处理正常人外周血单个核细胞（MN）可诱导抑制性细胞的产生。当存在经NaIO4预处理的细胞时，新鲜的同种异体和自体细胞对植物血凝素（PHA）、刀豆球蛋白A（Con A）、商陆有丝分裂原（PWM）、各种抗原以及混合淋巴细胞培养的促有丝分裂反应受到抑制。经NaIO4预处理的细胞也抑制PWM诱导的空斑形成细胞反应。在NaIO4处理之前用丝裂霉素C处理细胞可消除抑制活性。1: 的比例导致最强的抑制作用。来自经NaIO4预处理的细胞培养物的上清液没有抑制作用。
