蛋白质与新生大鼠小肠分离肠上皮细胞的结合。
The binding of proteins to isolated enterocytes from the small intestine of the neonatal rat.
作者信息
Mackenzie N M, Morris B, Morris R
出版信息
Immunology. 1983 Mar;48(3):489-96.
IgG binds specifically to isolated jejunal enterocytes but not to ileal enterocytes; maximum binding occurred at pH 6. The ability of jejunal enterocytes to bind IgG was reduced to low levels at 20 days of age and was lost at 24 days. Human and rat IgG were bound specifically in similar amounts; human IgG displaced rat IgG with identical efficiency to rat IgG (ED50 = 50 nM). Much less bovine and sheep IgG were bound to enterocytes and the ED50s for these proteins were 150 nM and 2.5 microM, respectively. Rat IgG bound to jejunal enterocytes with high affinity (13.21 x 10(6)M-1) and to 4.83 x 10(6) sites per cell. Receptor protein was estimated to represent 0.18% of total cell protein. These observations are discussed in relation to the results of in vivo IgG transmission studies. It is estimated that the IgG transport mechanism, operating at maximum efficiency, requires that available IgG receptors would come into use once to twice per hour.
IgG特异性结合分离的空肠肠上皮细胞，而不结合回肠肠上皮细胞；最大结合发生在pH 6时。空肠肠上皮细胞结合IgG的能力在20日龄时降至低水平，并在24日龄时丧失。人源和大鼠源IgG的特异性结合量相似；人源IgG取代大鼠源IgG的效率与大鼠源IgG相同（半数有效剂量[ED50]=50 nM）。与肠上皮细胞结合的牛源和羊源IgG要少得多，这些蛋白质的ED50分别为150 nM和2.5 μM。大鼠源IgG以高亲和力（13.21×10⁶ M⁻¹）结合空肠肠上皮细胞，每个细胞有4.83×10⁶个结合位点。估计受体蛋白占细胞总蛋白的0.18%。结合体内IgG转运研究的结果对这些观察结果进行了讨论。据估计，以最大效率运行的IgG转运机制要求可用的IgG受体每小时使用一到两次。
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