Stegmayr B, Ronquist G
Urol Res. 1982;10(5):253-7. doi: 10.1007/BF00255932.
Seminal plasma constituents were separated on Sephadex G200 gel columns. The column eluate was analysed with regard to protein content, ATPase activity and promotive activity on sperm progressive motility. Two different chromatographic fractions were also subjected to electron microscopy after sedimentation by preparative ultracentrifugation. A maximum promotive value on sperm progressive motility coincided with a maximum ATPase activity value in a single peak from seminal plasma eluted first on the column and containing less protein than the other peaks appearing later in the chromatogram. This first peak was the only one containing ATPase activity and membrane-surrounded organelles named prostasomes. Other peaks, rich in protein but lacking ATPase and prostasomes, displayed a moderate and rather irregular pattern in reference to promotive activity on sperm progressive motility. Evidence is given that the positive effect by prostasomes is specific on sperm progressive motility. Hence, procedures aiming at a change of membrane integrity of the prostasomes resulted in diminished effects on sperm progressive motility. This could be explained by a probable dissipation of the electrochemical gradient of calcium ions.
精液血浆成分在葡聚糖G200凝胶柱上进行分离。对柱洗脱液进行蛋白质含量、ATP酶活性以及对精子前向运动促进活性的分析。通过制备性超速离心沉淀后，对两个不同的色谱馏分也进行了电子显微镜检查。精液血浆在柱上首先洗脱的单个峰中，对精子前向运动的最大促进值与最大ATP酶活性值一致，且该峰中的蛋白质含量低于色谱图中随后出现的其他峰。这个第一个峰是唯一含有ATP酶活性和名为前列腺小体的膜包裹细胞器的峰。其他富含蛋白质但缺乏ATP酶和前列腺小体的峰，在对精子前向运动的促进活性方面呈现出中等且相当不规则的模式。有证据表明前列腺小体对精子前向运动的积极作用是特异性的。因此，旨在改变前列腺小体膜完整性的操作会导致对精子前向运动的影响减弱。这可以通过钙离子电化学梯度可能的消散来解释。
