Fabiani R, Johansson L, Lundkvist O, Ulmsten U, Ronquist G
Department of Clinical Chemistry, University Hospital, University of Uppsala, Sweden.
Eur J Obstet Gynecol Reprod Biol. 1994 Dec;57(3):181-8. doi: 10.1016/0028-2243(94)90298-4.
Prostasomes, small corpuscular organelles derived from the prostate gland, were isolated from human seminal plasma by means of ultracentrifugation and Sephadex G 200 chromatography to assess objectively their promotive effect on the motility of buffer-washed normal human spermatozoa exhibiting no forward motility. Prostasomes were efficacious in about 70% of these spermatozoa, and a maximum value was obtained with prostasomes at a concentration corresponding to a protein content of 0.7-0.8 g/l followed by a plateau at higher concentrations. Addition of albumin alone resulted in a similar response although at a somewhat lower level and about 50% of the spermatozoa were rendered motile with a maximum effect of albumin at about 2.5 g/l. Albumin concentrations exceeding 3 g/l were less active. At protein concentrations of 0.25 g/l, prostasomes were superior to albumin in every respect concerning the effects on various sperm movement characteristics. These divergent effects were abolished when comparing prostasomes corresponding to a protein concentration of 0.75 g/l with albumin at 2.3 g/l, i.e. at their respective optimum concentration. Heat treatment and ultrasonication of prostasomes did not affect their motility-promoting properties. Some problem of sperm dysfunction has generally been considered to be a major contributory factor to infertility. By supplementing sperm preparations with postasomes in cases of established male factor, the already poor quality spermatozoa may more frequently be rendered capable of fertilization after insemination.
前列腺小体是源自前列腺的小颗粒细胞器，通过超速离心和葡聚糖凝胶G 200色谱法从人精浆中分离出来，以客观评估其对经缓冲液洗涤后无向前运动能力的正常人精子运动的促进作用。前列腺小体对约70%的此类精子有效，当前列腺小体浓度对应蛋白质含量为0.7 - 0.8 g/l时可获得最大值，更高浓度时则保持平稳。单独添加白蛋白也会产生类似反应，不过程度稍低，约50%的精子可被激活，白蛋白在约2.5 g/l时效果最佳。白蛋白浓度超过3 g/l时活性降低。在蛋白质浓度为0.25 g/l时，前列腺小体在影响各种精子运动特征方面在各方面均优于白蛋白。当比较蛋白质浓度为0.75 g/l的前列腺小体与2.3 g/l的白蛋白（即各自的最佳浓度）时，这些不同的作用消失。对前列腺小体进行热处理和超声处理不影响其促进运动的特性。精子功能障碍的某些问题通常被认为是导致不孕的主要因素。在已确定存在男性因素的情况下，通过在精子制剂中补充前列腺小体，质量本就不佳的精子在授精后更有可能具备受精能力。
