Barnett M A, Learmonth R P, Pihl E, Wood E C
J Reprod Immunol. 1983 Jan;5(1):55-7. doi: 10.1016/0165-0378(83)90021-9.
Peripheral blood lymphocyte subclasses were determined in 60 women with normal pregnancies, 20 from each trimester, and in 20 controls using automated flow cytofluorimetry. The cells were stained with the monoclonal antibodies OKT3, OKT4 and OKT8 to stain total T cells, T helper and T suppressor-cytotoxic lymphocytes, respectively. A polyvalent rabbit anti-human Ig serum was used to stain B lymphocytes. Absolute numbers of T lymphocytes were significantly reduced in both the first and second trimesters. This was due to a significant decrease in T helper lymphocytes and a smaller, statistically not significant, reduction in the number of T suppressor lymphocytes. There was no significant change in lymphocyte subclasses during the third trimester. Total lymphocyte numbers were normal throughout pregnancy.
采用自动流式细胞荧光测定法，对60名正常妊娠女性（每个孕期20名）及20名对照者的外周血淋巴细胞亚群进行了测定。细胞分别用单克隆抗体OKT3、OKT4和OKT8染色，以分别标记总T细胞、T辅助细胞和T抑制-细胞毒性淋巴细胞。用多价兔抗人Ig血清标记B淋巴细胞。在妊娠的第一和第二阶段，T淋巴细胞的绝对数量显著减少。这是由于T辅助淋巴细胞数量显著减少，而T抑制淋巴细胞数量减少幅度较小，在统计学上无显著意义。在妊娠第三阶段，淋巴细胞亚群无显著变化。整个孕期淋巴细胞总数正常。
