Sakaguchi S
Microbiol Immunol. 1982;26(11):1017-34. doi: 10.1111/j.1348-0421.1982.tb00251.x.
A study was performed to clarify the role of serum lipoproteins, especially high density lipoprotein (HDL) and triglyceride-rich lipoproteins in endotoxemic or endotoxin-poisoned animals. The level of HDL-cholesterol decreased markedly in mouse serum 18-24 hr postintoxication, while the amount of low density lipoprotein (LDL)-cholesterol in the sera of poisoned mice was about 175% of that of the controls. Serum lecithin-cholesterol acyltransferase activity in the poisoned mice decreased slightly for 3-6 hr after endotoxin injection, but became markedly increased at 18-24 hr as compared with that in the controls. The amount of serum very low density lipoprotein (VLDL) showed a marked increase in the poisoned mice 8-24 hr postintoxication. The HDL fraction in the electrophoretic patterns of serum was reduced according to the dose of endotoxin 18 hr postintoxication. The HDL fraction in mice injected with lead acetate plus endotoxin was markedly lower than that in the poisoned mice. When streptozotocin-diabetic mice were injected with endotoxin, the HDL fraction was higher than that in the endotoxin-poisoned mice. In endotoxin-poisoned mice a correlation was observed between the lipid peroxide and LDL levels in the serum. In disk electrophoretic patterns, the HDL fraction in mice given vitamin E-supplemented diet showed a higher level than that in mice given a normal diet. Lipoprotein lipase (LPL) activity in poisoned mice significantly decreased to 59% of the control value 18 hr postintoxication, but hepatic triglyceride lipase activity was only slightly increased in endotoxin-poisoned mice. In analysis of HDL apoprotein peptide in serum lipoprotein, the apo C-II peptide level was clearly lower in mouse serum 18 hr postintoxication than that in the controls. These results suggest that the decrease in LPL activity in endotoxin-poisoned mice may be closely related to a decrease in the apo C-II peptide level, and also that it plays an important part in HDL and triglyceride-rich lipoprotein metabolism in the poisoned mice.
进行了一项研究，以阐明血清脂蛋白，尤其是高密度脂蛋白（HDL）和富含甘油三酯的脂蛋白在内毒素血症或内毒素中毒动物中的作用。中毒后18 - 24小时，小鼠血清中HDL胆固醇水平显著下降，而中毒小鼠血清中低密度脂蛋白（LDL）胆固醇含量约为对照组的175%。内毒素注射后3 - 6小时，中毒小鼠血清卵磷脂胆固醇酰基转移酶活性略有下降，但与对照组相比，在18 - 24小时时显著升高。中毒小鼠中毒后8 - 24小时，血清极低密度脂蛋白（VLDL）含量显著增加。中毒后18小时，血清电泳图谱中的HDL部分根据内毒素剂量而减少。注射醋酸铅加内毒素的小鼠的HDL部分明显低于中毒小鼠。当链脲佐菌素诱导的糖尿病小鼠注射内毒素时，HDL部分高于内毒素中毒小鼠。在内毒素中毒小鼠中，观察到血清中脂质过氧化物与LDL水平之间存在相关性。在圆盘电泳图谱中，给予补充维生素E饮食的小鼠的HDL部分水平高于给予正常饮食的小鼠。中毒小鼠中毒后18小时，脂蛋白脂肪酶（LPL）活性显著下降至对照值的59%，但内毒素中毒小鼠的肝甘油三酯脂肪酶活性仅略有增加。在分析血清脂蛋白中的HDL载脂蛋白肽时，中毒后18小时小鼠血清中的载脂蛋白C-II肽水平明显低于对照组。这些结果表明，内毒素中毒小鼠中LPL活性的降低可能与载脂蛋白C-II肽水平的降低密切相关，并且在中毒小鼠的HDL和富含甘油三酯的脂蛋白代谢中起重要作用。
