Banga J P, Hutchings P, Tatham P E, Lang I, Gunn H, Cooke A, Roitt I M
Cell Immunol. 1983 Jun;78(2):285-94. doi: 10.1016/0008-8749(83)90283-6.
Mixed-lymphocyte culture-stimulated cells have been fractionated by their ability to bind the lectin Vicia villosa (Vv) and assessed for their cytolytic and suppressor activity in vitro. Vv positive and negative cells were separated either by cell affinity chromatography using Vv-Sepharose 6MB chromatography or by electronic cell sorting with FITC-Vv. Both populations expressed marked cytolytic and suppressor cell activity. Thus this lectin cannot be used to discriminate between these and other functional lymphoid cell population of blastoid cells binding the FITC-Vv appears following allogeneic stimulation; treatment with the immunosuppressive drug cyclosporin A, which affects cytotoxic cells preferentially, results in a considerable reduction of the Vv positive blastoid cells.
混合淋巴细胞培养刺激的细胞已根据其结合凝集素野豌豆（Vv）的能力进行了分级，并在体外评估了它们的细胞溶解和抑制活性。通过使用Vv-琼脂糖6MB色谱的细胞亲和色谱法或通过用异硫氰酸荧光素-Vv进行电子细胞分选来分离Vv阳性和阴性细胞。这两个群体均表现出明显的细胞溶解和抑制细胞活性。因此，这种凝集素不能用于区分这些细胞与其他结合异硫氰酸荧光素-Vv的胚样细胞功能性淋巴细胞群体；用优先影响细胞毒性细胞的免疫抑制药物环孢菌素A处理，会导致Vv阳性胚样细胞数量大幅减少。
