Braciale V L, Friedman H P, Braciale T J
J Immunol Methods. 1981;43(3):241-50. doi: 10.1016/0022-1759(81)90171-x.
We describe a simple procedure for isolation of Vicia villosa lectin with binding specificity for N-acetyl-D-galactosamine greater than D-galactose. Also, a rosette assay using V. villosa lectin coupled-SRBC is described which enables us to both visualize lectin binding cells and to fractionate lymphocytes from vitro primary mixed lymphocyte cultures on the basis of ability to bind V. villosa lectin.
我们描述了一种简单的程序，用于分离对N-乙酰-D-半乳糖胺的结合特异性大于D-半乳糖的野豌豆凝集素。此外，还描述了一种使用偶联有野豌豆凝集素的绵羊红细胞进行的玫瑰花结试验，该试验使我们既能观察到凝集素结合细胞，又能根据结合野豌豆凝集素的能力从体外原发性混合淋巴细胞培养物中分离淋巴细胞。
