伴刀豆球蛋白A可导致猪淋巴细胞的钠通透性增加和细胞内钠含量升高。
Concanavalin A causes an increase in sodium permeability and intracellular sodium content of pig lymphocytes.
作者信息
Felber S M, Brand M D
出版信息
Biochem J. 1983 Mar 15;210(3):893-7. doi: 10.1042/bj2100893.
Abstract
- The 3mV depolarization of pig lymphocytes observed within 2 1/2 min of treatment with concanavalin A [Felber & Brand (1983) Biochem. J. 210, 885-891] is dependent on the presence of high extracellular [Na+]. 2. The concanavalin A-induced changes in membrane potential at high and low extracellular [Na+] are quantitatively explained by an increase in the electrogenic permeability coefficient for Na+ (PNa). This rises from a negligible value in resting cells to around 4% of the permeability coefficient for K+ or Cl- in stimulated cells. 3. Concanavalin A induces a 4mM increase in the Na+ content of pig lymphocytes. This increase in intracellular [Na+] is not due solely to stimulation of electrogenic Na+ influx resulting from the rise in PNa. 4. Thus concanavalin A stimulates both an electrogenic pathway for Na+ influx, resulting in a small depolarization of the plasma membrane, and a non-electrogenic Na+ influx pathway, resulting in a rise in intracellular [Na+].
摘要
- 在用伴刀豆球蛋白A处理猪淋巴细胞2.5分钟内观察到的3毫伏去极化现象[费尔伯和布兰德（1983年），《生物化学杂志》210卷，885 - 891页]依赖于高细胞外[Na⁺]的存在。2. 伴刀豆球蛋白A在高细胞外[Na⁺]和低细胞外[Na⁺]情况下引起的膜电位变化，通过Na⁺的生电通透性系数（PNa）增加得到了定量解释。它从静息细胞中的可忽略不计的值上升到刺激细胞中K⁺或Cl⁻通透性系数的约4%。3. 伴刀豆球蛋白A使猪淋巴细胞的Na⁺含量增加4毫摩尔。细胞内[Na⁺]的这种增加并非仅仅由于PNa升高导致的生电Na⁺内流受到刺激。4. 因此，伴刀豆球蛋白A既刺激了导致质膜轻微去极化的生电Na⁺内流途径，也刺激了导致细胞内[Na⁺]升高的非生电Na⁺内流途径。
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