决定淋巴细胞质膜电位的因素。
Factors determining the plasma-membrane potential of lymphocytes.
作者信息
Felber S M, Brand M D
出版信息
Biochem J. 1982 May 15;204(2):577-85. doi: 10.1042/bj2040577.
Abstract
- Lymphocytes from pig mesenteric lymph node have low permeability to K+ (Rb+), Na+ and Cl-. None of these ions is in Nernst equilibrium with the plasma-membrane potential (delta psi p). 2. delta psi p can be calculated from the transmembrane distribution of the permeant cation methyltriphenylphosphonium (TPMP+) in the presence of the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) to abolish uptake into intracellular mitochondria. In normal culture medium delta psi p is 56 mV. 3. A similar potential is found in T-enriched pig cells and in mouse thymocytes. 4. The contribution of electrogenic (Na+ + K+)ATPase to delta psi p is about 7 mV. 5. The remainder of the lymphocyte delta psi p is a polyionic potential set up by K+ and Cl- with a permeability coefficient for Cl- of similar magnitude to that for K+.
摘要
- 猪肠系膜淋巴结中的淋巴细胞对K⁺（Rb⁺）、Na⁺和Cl⁻具有低通透性。这些离子均未与质膜电位（δψp）处于能斯特平衡。2. 在解偶联剂羰基氰对三氟甲氧基苯腙（FCCP）存在的情况下，可根据通透阳离子甲基三苯基鏻（TPMP⁺）的跨膜分布计算δψp，以消除其摄入细胞内线粒体。在正常培养基中，δψp为56 mV。3. 在富含T细胞的猪细胞和小鼠胸腺细胞中发现了类似的电位。4. 电生性（Na⁺ + K⁺）ATP酶对δψp的贡献约为7 mV。5. 淋巴细胞δψp的其余部分是由K⁺和Cl⁻建立的多离子电位，Cl⁻的通透系数与K⁺的通透系数大小相似。
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