速尿对伴刀豆球蛋白A诱导的淋巴细胞细胞膜通透性增加的特性研究

Characterization of the concanavalin A-induced increase in lymphocyte cell membrane permeability by furosemide.

作者信息

Averdunk R, Günther T

出版信息

Immunobiology. 1980 Dec;157(4-5):358-64. doi: 10.1016/S0171-2985(80)80005-2.

Abstract

The effect of ConA on passive 42K+ fluxes in human lymphocytes was analysed by treatment with furosemide and ouabain. Furosemide has no effect on net K+ transport and pump flux. The component of passive efflux of 42K+ which depends on K+-K+ exchange is inhibited by furosemide; its mechanism is discussed in detail. The 42K+ uptake in lymphocytes consists of 1. an ouabain-sensitive pump influx, 2. an ouabain-insensitive leak influx and 3. a furosemide-sensitive K+-K+-exchange. Only the first two components of 42K+ uptake are stimulated by ConA.

摘要

通过用速尿和哇巴因处理，分析了刀豆球蛋白A对人淋巴细胞中被动42K⁺通量的影响。速尿对净K⁺转运和泵通量无影响。依赖于K⁺-K⁺交换的42K⁺被动外流成分被速尿抑制；详细讨论了其机制。淋巴细胞对42K⁺的摄取包括：1. 哇巴因敏感的泵内流；2. 哇巴因不敏感的渗漏内流；3. 速尿敏感的K⁺-K⁺交换。刀豆球蛋白A仅刺激42K⁺摄取的前两个成分。