Naganuma A, Imura N
J Pharmacobiodyn. 1983 May;6(5):331-9. doi: 10.1248/bpb1978.6.331.
In vitro interaction profiles of mercuric mercury and selenite in rabbit blood under various reaction conditions were investigated. The most remarkable changes in the incorporations into the erythrocytes and gel filtration profiles of mercury and selenium in blood were observed when 10(-5) M mercuric chloride and 10(-5) M selenite were added to rabbit blood. Under this conditions, the mercury and selenium added always behaved with each other on gel filtration suggesting that most of mercury and selenium existed in a complex at a molar ratio of 1. When the concentration of mercuric chloride was 10(-7) in blood, however, the incorporation of mercury into erythrocytes was also increased by the simultaneous addition of selenite, but 10(-7) mercury did not stimulate the selenium incorporation. The behaviors of mercury and selenium in blood were altered by the order of addition of mercuric chloride and selenite to the blood. Compared to the simultaneous addition of both compounds, the final amount of mercury and selenium incorporated into erythrocytes were reduced by the addition of mercuric chloride prior to selenite, whereas the rates of incorporation of mercury and selenium were lowered by the addition of selenite prior to mercury. The gel filtration patterns of mercury and selenium in the plasma and stroma-free hemolysate of the blood preincubated with selenite before the addition of mercury were different from the case of simultaneous addition of both compounds or addition of mercury prior to selenite. The variety of interaction profiles of mercuric mercury and selenium in blood under different reaction conditions as observed in the present in vitro study may reflect the complex modes of interaction actually occurs in vivo.
研究了不同反应条件下兔血中汞离子和亚硒酸盐的体外相互作用情况。当向兔血中加入10⁻⁵M氯化汞和10⁻⁵M亚硒酸盐时，观察到血液中汞和硒在红细胞中的掺入情况以及凝胶过滤图谱发生了最显著的变化。在此条件下，添加的汞和硒在凝胶过滤中总是相互作用，表明大部分汞和硒以1:1的摩尔比形成复合物存在。然而，当血液中氯化汞的浓度为10⁻⁷时，同时添加亚硒酸盐也会增加汞在红细胞中的掺入，但10⁻⁷的汞不会刺激硒的掺入。血液中汞和硒的行为会因向血液中添加氯化汞和亚硒酸盐的顺序而改变。与同时添加两种化合物相比，先添加氯化汞再添加亚硒酸盐会使最终掺入红细胞的汞和硒的量减少，而先添加亚硒酸盐再添加汞则会降低汞和硒的掺入速率。在添加汞之前先用亚硒酸盐预孵育的血液的血浆和无基质溶血产物中，汞和硒的凝胶过滤模式与同时添加两种化合物或先添加汞的情况不同。本体外研究中观察到的不同反应条件下血液中汞离子和硒的多种相互作用情况可能反映了体内实际发生的复杂相互作用模式。
