Alavi M, Dunnett C W, Moore S
Arteriosclerosis. 1983 Sep-Oct;3(5):413-9. doi: 10.1161/01.atv.3.5.413.
To determine the lipid content of the neointima formed following balloon catheter deendothelialization, we measured the chemical composition of intimal medial samples of the aortic wall of rabbits at 6, 12, and 24 months after the operation. The areas of endothelial regrowth and the denuded areas were outlined by intravenous injection of Evan's blue dye. Lipids were extracted by chloroform/methanol (2:1, vol/vol) separated by thin-layer chromatography, eluted, and estimated by gas-liquid chromatography. No tendency to regression was observed. By contrast we observed a progressive increase over time in the concentration of most lipid classes in the areas of endothelial regrowth. There is evidence indicating that the increase in the lipid concentration parallels the increase in the glycosaminoglycan content in the areas of endothelial regrowth. It is possible that endothelial injury causes some irreversible changes in the composition of the neointima in areas of endothelial regeneration.
为了确定球囊导管去内皮后形成的新生内膜的脂质含量，我们在术后6个月、12个月和24个月测量了兔主动脉壁内膜中层样本的化学成分。通过静脉注射伊文思蓝染料勾勒出内皮再生区域和剥脱区域。脂质用氯仿/甲醇（2:1，体积/体积）提取，通过薄层色谱分离、洗脱，并通过气液色谱法进行估计。未观察到消退趋势。相比之下，我们观察到在内皮再生区域，大多数脂质类别的浓度随时间逐渐增加。有证据表明，脂质浓度的增加与内皮再生区域糖胺聚糖含量的增加平行。内皮损伤可能在内皮再生区域的新生内膜组成中引起一些不可逆的变化。
