Vigh B, Vigh-Teichmann I, Röhlich P, Oksche A
Cell Tissue Res. 1983;233(3):539-48. doi: 10.1007/BF00212223.
Opsin-immunoreactive sites of hypothalamic cerebrospinal fluid (CSF)-contacting neurons, pinealocytes and retinal cells were studied in various vertebrates (Carassius auratus, Phoxinus phoxinus, Triturus cristatus, Bombina bombina, Rana esculenta) by means of postembedding immuno-electron microscopy with the use of the protein A-gold labeling method. The retina of the rat served as a general reference tissue for the quality of the immunocytochemical reaction. A strong opsin immunoreaction (rat-antibovine opsin serum) was obtained in the rod-type outer segments of photoreceptors in the retina of all species studied. Cone-type outer segments exhibited only very few antigenic binding sites. In the pineal organ of the goldfish and the frog, outer segments of the photoreceptor cells displayed strong immunoreactivity. No immunoreaction was found in hypothalamic CSF-contacting neurons and Landolt's clubs of nerve cells of the bipolar layer of the retina. The morphological similarity between the ciliated dendritic terminal of the Landolt's club and the intraventricular dendritic ending of the CSF-contacting neurons is emphasized.
利用蛋白A-金标记法的包埋后免疫电子显微镜技术，在多种脊椎动物（鲫鱼、赤睛鳟、火蝾螈、东方铃蟾、食用蛙）中研究了下丘脑脑脊液（CSF）接触神经元、松果体细胞和视网膜细胞的视蛋白免疫反应位点。大鼠视网膜用作免疫细胞化学反应质量的通用参照组织。在所研究的所有物种的视网膜中，光感受器的杆状型外段均获得了强烈的视蛋白免疫反应（大鼠抗牛视蛋白血清）。锥状型外段仅显示出极少的抗原结合位点。在金鱼和青蛙的松果体器官中，光感受器细胞的外段表现出强烈的免疫反应性。在下丘脑CSF接触神经元和视网膜双极层神经细胞的郎氏小体中未发现免疫反应。强调了郎氏小体的纤毛树突末端与CSF接触神经元的脑室内树突末端之间的形态学相似性。
