van Veen T, Ekström P, Nyberg L, Borg B, Vigh-Teichmann I, Vigh B
Cell Tissue Res. 1984;237(3):559-64. doi: 10.1007/BF00228440.
5-hydroxytryptamine (5-HT, serotonin)- and opsin-immunoreactive sites were studied in the developing pineal complex of the stickleback, Gasterosteus aculeatus L., by use of light-microscopic indirect immunoperoxidase techniques. 5-HT immunoreactivity first occurs in the pineal organ at the age of 80 h after fertilization and appears to be localized in cells of the photoreceptor type. The outer segments of a few pineal photosensory cells exhibit opsin immunoreactivity at the age of 84 h after fertilization. The number of cells seems to increase until the pineal organ is completely developed. The increase in the number of 5-HT immunoreactive perikarya runs parallel in time to that of the opsin-immunoreactive outer segments. The cells of the parapineal organ show neither opsin nor 5-HT immunoreactivity. The retina of the embryonic stickleback does not display opsin immunoreactivity until after hatching, which takes place about 144 h after fertilization. These results suggest, in the three-spined stickleback, an earlier light-perception capacity for the developing pineal organ than for the retina.
利用光学显微镜间接免疫过氧化物酶技术，对棘背鱼（Gasterosteus aculeatus L.）发育中的松果体复合体中的5-羟色胺（5-HT，血清素）和视蛋白免疫反应位点进行了研究。5-HT免疫反应性在受精后80小时首次出现在松果体器官中，似乎定位于光感受器类型的细胞中。在受精后84小时，少数松果体光感细胞的外段表现出视蛋白免疫反应性。细胞数量似乎一直增加，直到松果体器官完全发育。5-HT免疫反应性核周体数量的增加在时间上与视蛋白免疫反应性外段的增加平行。副松果体器官的细胞既不显示视蛋白也不显示5-HT免疫反应性。胚胎棘背鱼的视网膜直到孵化后才显示视蛋白免疫反应性，孵化大约在受精后144小时发生。这些结果表明，在三刺棘背鱼中，发育中的松果体器官比视网膜具有更早的光感知能力。
