Tauris P, Nielsen J L
Acta Pathol Microbiol Immunol Scand C. 1983 Aug;91(4):257-61.
The in vitro immunoglobulin (Ig) secretion of pokeweed mitogen (PWM)-activated peripheral blood lymphocytes (PBL) from individuals splenectomized post-trauma was monitored with a protein A plaque-forming cell (PFC) assay. Cultures of unfractionated as well as reconstituted cultures of isolated erythrocyte rosette-forming (E-RFC)-positive (T lymphocytes) and E-RFC-negative (B lymphocytes) cells were established. Using unfractionated cells, the response was substantially reduced or absent, whereas cultures of autologous untreated B and 2000 rads irradiated T cells restored the response to normal levels. Normal T cells were not able to stimulate patients' B cells to Ig-secretion and patients' untreated T cells did not induce plaque formation in normal B cells, whereas irradiated patients' T cells induced development of approximately 50% of the response induced by normal irradiated T cells. These results indicate that the immunological defect in splenectomized individuals is not merely restricted to a high level of radiosensitive T cell suppression but also involves an impaired B cell function and T/B cell cooperation.
采用蛋白A斑形成细胞（PFC）试验监测创伤后脾切除个体的商陆丝裂原（PWM）激活的外周血淋巴细胞（PBL）的体外免疫球蛋白（Ig）分泌情况。建立了未分级细胞培养以及分离的红细胞花环形成（E-RFC）阳性（T淋巴细胞）和E-RFC阴性（B淋巴细胞）细胞的重组培养。使用未分级细胞时，反应显著降低或不存在，而自体未处理的B细胞和2000拉德照射的T细胞培养可将反应恢复至正常水平。正常T细胞无法刺激患者B细胞分泌Ig，患者未处理的T细胞也不能诱导正常B细胞形成斑块，而照射后的患者T细胞诱导的反应约为正常照射T细胞诱导反应的50%。这些结果表明，脾切除个体的免疫缺陷不仅限于高水平的放射敏感T细胞抑制，还涉及B细胞功能受损以及T/B细胞协作障碍。
