Brewer J A, Hank J A, Wendel T, Schmeling G J, Blank J L, Morrissey L W, Robins H I, Sondel P M
Tissue Antigens. 1983 Oct;22(4):246-56. doi: 10.1111/j.1399-0039.1983.tb01200.x.
We have utilized serological techniques and mixed lymphocyte culture (MLC) reactions to examine HLA-DR and HLA-D expression by heated (45 degrees C for 1 h) lymphocytes in order to study the functional relationship of these antigens. Heated lymphocytes do not stimulate proliferation of allogeneic lymphocytes in MLC, yet they express HLA-DR antigens. The fraction of peripheral blood lymphocytes (PBL) expressing DR is not altered by heating, nor is the staining intensity altered as detected by fluorescence microscopy. Alloantisera to "B cell alloantigens" recognize HLA-DR determinants on heated cells without any detectable change in either specificity or quantitative cytotoxic effects. Flow cytometry with monoclonal antibody demonstrates only minimal decrease in HLA-DR expression after heating. Thus stimulation in MLC requires more of the stimulating cell than the mere expression of HLA-DR.
我们运用血清学技术和混合淋巴细胞培养（MLC）反应，检测经加热（45℃ 1小时）的淋巴细胞上HLA - DR和HLA - D的表达，以研究这些抗原的功能关系。加热的淋巴细胞在MLC中不会刺激同种异体淋巴细胞增殖，但它们表达HLA - DR抗原。表达DR的外周血淋巴细胞（PBL）比例不会因加热而改变，通过荧光显微镜检测，染色强度也未改变。针对“B细胞同种异体抗原”的同种抗血清可识别加热细胞上的HLA - DR决定簇，特异性或定量细胞毒性效应均未出现可检测到的变化。用单克隆抗体进行的流式细胞术显示，加热后HLA - DR表达仅略有下降。因此，MLC中的刺激需要的刺激细胞条件比单纯表达HLA - DR更多。
