Holoshitz J, Matitiau A, Cohen I R
J Clin Invest. 1984 Jan;73(1):211-5. doi: 10.1172/JCI111193.
We have been studying the pathogenesis of adjuvant arthritis in rats using a long-term cell line of T lymphocytes, the A2 line, which can induce polyarthritis and can also be used to vaccinate rats against adjuvant arthritis. Although line A2 was selected for its proliferative response to mycobacteria, it also responded to collagen type II. To elucidate its role of responsiveness to collagen type II and the relationship between arthritogenicity and vaccination, we cloned A2 and selected a subline A2b. We now report that subline A2b, which bore a marker of helper/delayed hypersensitivity T lymphocytes, was strongly arthritogenic, but could not vaccinate against arthritis. Moreover, A2b showed no response to collagen type II. Therefore, reactivity to collagen type II is not a requisite for arthritogenicity, and mediation of arthritis and vaccination can be distinct properties of different populations of T lymphocytes.
我们一直在使用一种T淋巴细胞长期细胞系——A2系来研究大鼠佐剂性关节炎的发病机制。该细胞系可诱发多关节炎，也可用于给大鼠接种疫苗以预防佐剂性关节炎。尽管选择A2系是因其对分枝杆菌的增殖反应，但它对II型胶原也有反应。为阐明其对II型胶原反应性的作用以及致关节炎性与疫苗接种之间的关系，我们克隆了A2系并筛选出一个亚系A2b。我们现在报告，带有辅助/迟发型超敏T淋巴细胞标志物的亚系A2b具有很强的致关节炎性，但不能预防关节炎。此外，A2b对II型胶原无反应。因此，对II型胶原的反应性不是致关节炎性的必要条件，关节炎的介导和疫苗接种可能是不同T淋巴细胞群体的不同特性。
