通过等电聚焦对变形链球菌细胞外糖基转移酶进行的比较研究。

Comparative study of Streptococcus mutans extracellular glycosyltransferases by isoelectric focusing.

作者信息

Tsumori H, Shimamura A, Mukasa H

出版信息

J Gen Microbiol. 1983 Oct;129(10):3261-9. doi: 10.1099/00221287-129-10-3261.

Abstract

Extracellular glycosyltransferases from 17 strains of Streptococcus mutans were examined by analytical isoelectric focusing. Three kinds of glucosyltransferase: highly-branched-1,6-alpha-D-glucan synthetase, 1,3-alpha-D-glucan synthetase and 1,6-alpha-D-glucan synthetase, were excreted from serotype a, d and g strains. The enzymes of serotype a strains were distinguishable from those of serotypes d and g by differences in their pI values. Serotype c, e and f strains excreted basic glucosyltransferase and acidic fructosyltransferase. Serotype b strains also excreted the glucosyl- and fructosyltransferases, but the pI values were different from those of the enzymes from the other serotypes. Thus, S. mutans strains could be divided into four groups by analytical isoelectric focusing of glycosyltransferases which corresponded well to the four genetic groups.

摘要

通过分析等电聚焦法检测了17株变形链球菌的细胞外糖基转移酶。从a、d和g血清型菌株中分泌出三种葡糖基转移酶：高度分支的1,6-α-D-葡聚糖合成酶、1,3-α-D-葡聚糖合成酶和1,6-α-D-葡聚糖合成酶。a血清型菌株的酶通过其pI值的差异与d和g血清型的酶区分开来。c、e和f血清型菌株分泌碱性葡糖基转移酶和酸性果糖基转移酶。b血清型菌株也分泌葡糖基和果糖基转移酶，但pI值与其他血清型的酶不同。因此，通过糖基转移酶的分析等电聚焦法，变形链球菌菌株可分为四组，这与四个基因组很好地对应。