Shimamura A, Tsumori H, Mukasa H
FEBS Lett. 1983 Jun 27;157(1):79-84. doi: 10.1016/0014-5793(83)81120-x.
In addition to the 1,3-alpha-D-glucan synthetase (pI 4.9) and the highly-branched 1,6-alpha-D-glucan synthetase (pI 3.9-4.1), Streptococcus mutans 6715 (serotype g) was found to secrete the third glucosyltransferase in multiple forms (pI 5.5-7.0), which exhibited 87% 1,6-alpha-bond-, 6% 1,3-alpha-bond- and 7% 1,3,6-branch-forming activities. The production of this enzyme was extremely enhanced when the organism was grown in Tween 80-supplemented medium. The 3 glucosyltransferases from the same organism were enzymatically and immunologically distinct from each other, and they were commonly found among the serotype g strains.
除了1,3-α-D-葡聚糖合成酶（pI 4.9）和高度分支的1,6-α-D-葡聚糖合成酶（pI 3.9 - 4.1）外，变形链球菌6715（血清型g）还被发现以多种形式分泌第三种葡糖基转移酶（pI 5.5 - 7.0），该酶表现出87%的1,6-α-键形成活性、6%的1,3-α-键形成活性和7%的1,3,6-分支形成活性。当该生物体在添加吐温80的培养基中生长时，这种酶的产量会极大提高。来自同一生物体的这3种葡糖基转移酶在酶学和免疫学上彼此不同，并且它们在血清型g菌株中普遍存在。
