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Monoclonal antibodies to the extracellular glucosyltransferases from Streptococcus sobrinus 6715.

作者信息

McCabe M M, Alberts M, Stein J

出版信息

Infect Immun. 1987 Aug;55(8):1900-5. doi: 10.1128/iai.55.8.1900-1905.1987.

DOI:10.1128/iai.55.8.1900-1905.1987
PMID:2956196
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC260622/
Abstract

Murine monoclonal antibodies (MAbs) were raised against the glucosyltransferases (GTFs) of Streptococcus sobrinus 6715. The antibody panels included MAbs raised against the primer-independent, soluble product enzyme (GTF-Si) which did not cross-react with other GTFs, as well as MAbs raised against the primer-dependent, soluble product enzyme (GTF-Sd) which recognized both GTF-Si and GTF-Sd, thus indicating that these catalytically distinct enzymes share epitopes. MAbs raised against GTF-I recognized several forms of GTF-I and did not cross-react with the GTF-S enzymes. None of the MAbs recognized the major glucan-binding protein of S. sobrinus. Two MAbs inhibited glucan synthesis, one blocking primer synthesis by GTF-Si by 89% and the second inhibiting that by GTF-I by 92%.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/8e121188a02f/iai00092-0175-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/9f35dbb6b425/iai00092-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/a6c2c60262be/iai00092-0174-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/b9b554db066c/iai00092-0174-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/a622bed727ca/iai00092-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/8e121188a02f/iai00092-0175-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/9f35dbb6b425/iai00092-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/a6c2c60262be/iai00092-0174-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/b9b554db066c/iai00092-0174-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/a622bed727ca/iai00092-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f29c/260622/8e121188a02f/iai00092-0175-b.jpg

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引用本文的文献

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本文引用的文献

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An endodextranase inhibitor from batch cultures of streptococcus mutans.来自变形链球菌分批培养物的一种内切葡聚糖酶抑制剂。
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Purification of a fourth glucosyltransferase from Streptococcus sobrinus.从远缘链球菌中纯化出第四种葡萄糖基转移酶。
J Bacteriol. 1989 Nov;171(11):6265-70. doi: 10.1128/jb.171.11.6265-6270.1989.
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Glucan-binding domain of a glucosyltransferase from Streptococcus sobrinus: isolation of a 55-kilodalton peptide from a trypsin digest of glucosyltransferase prebound to insoluble glucan.来自远缘链球菌的葡糖基转移酶的葡聚糖结合结构域：从预先结合到不溶性葡聚糖上的葡糖基转移酶的胰蛋白酶消化产物中分离出一个55千道尔顿的肽段。
Infect Immun. 1989 Jul;57(7):2210-3. doi: 10.1128/iai.57.7.2210-2213.1989.
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Identification of mutans streptococci with monoclonal antibodies.用单克隆抗体鉴定变形链球菌
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Microbiol Rev. 1980 Jun;44(2):331-84. doi: 10.1128/mr.44.2.331-384.1980.
4
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.“蛋白质免疫印迹法”：蛋白质从十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳转移至未修饰的硝酸纤维素膜上，并用抗体和放射性碘化蛋白A进行放射自显影检测。
Anal Biochem. 1981 Apr;112(2):195-203. doi: 10.1016/0003-2697(81)90281-5.
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The origin and composition of multiple forms of dextransucrase from Streptococcus sanguis.
Biochim Biophys Acta. 1984 Apr 27;786(1-2):42-8. doi: 10.1016/0167-4838(84)90151-1.
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J Gen Microbiol. 1983 Oct;129(10):3261-9. doi: 10.1099/00221287-129-10-3261.
7
Production of monoclonal antibody against a glucosyltransferase of Streptococcus mutans 6715.抗变形链球菌6715葡糖基转移酶单克隆抗体的制备
Infect Immun. 1983 Aug;41(2):872-5. doi: 10.1128/iai.41.2.872-875.1983.
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Three kinds of extracellular glucosyltransferases from Streptococcus mutans 6715 (serotype g).变形链球菌6715（血清型g）的三种细胞外葡糖基转移酶
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Protein transfer from isoelectric focusing Gels: the native blot.
Anal Biochem. 1982 Jul 1;123(2):229-35. doi: 10.1016/0003-2697(82)90439-0.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.