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导入小鼠L细胞的猪主要组织相容性DNA序列的差异表达

Differential expression of porcine major histocompatibility DNA sequences introduced into mouse L cells.

作者信息

Satz M L, Singer D S

出版信息

Mol Cell Biol. 1983 Nov;3(11):2006-16. doi: 10.1128/mcb.3.11.2006-2016.1983.

DOI:10.1128/mcb.3.11.2006-2016.1983
PMID:6228718
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC370068/
Abstract

The expression of a porcine genomic DNA segment containing a major histocompatibility gene and its chromatin structure in mouse L cells have been investigated. The transformed cells, which contain about two copies of the 17.8-kilobase pig DNA insert per haploid genome, stably and uniformly express major histocompatibility antigen on their surfaces. This expression is the result of differential transcription of the 3-kilobase major histocompatibility gene; the other 14 kilobases of pig sequences flanking the coding sequence are not transcribed. Although the entire pig DNA segment is packaged into nucleosomes, only the transcriptionally active DNA sequences are packaged in a DNase I-sensitive conformation. These results suggest that the expression of this foreign DNA is actively regulated in L cells.

摘要

对含有主要组织相容性基因的猪基因组DNA片段及其在小鼠L细胞中的染色质结构进行了研究。转化细胞每个单倍体基因组含有约两个拷贝的17.8千碱基猪DNA插入片段，在其表面稳定且均匀地表达主要组织相容性抗原。这种表达是3千碱基主要组织相容性基因差异转录的结果；编码序列两侧的另外14千碱基猪序列不转录。尽管整个猪DNA片段被包装成核小体，但只有转录活性的DNA序列以对DNase I敏感的构象包装。这些结果表明，这种外源DNA的表达在L细胞中受到积极调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/b33cdbcdb096/molcellb00111-0131-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/b069e5464733/molcellb00111-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/c6be444cd249/molcellb00111-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/3599e051d033/molcellb00111-0128-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/60d4a9b42d33/molcellb00111-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/505b812aeaa5/molcellb00111-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/84ddee3b233d/molcellb00111-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/b33cdbcdb096/molcellb00111-0131-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/b069e5464733/molcellb00111-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/c6be444cd249/molcellb00111-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/3599e051d033/molcellb00111-0128-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/60d4a9b42d33/molcellb00111-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/505b812aeaa5/molcellb00111-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/84ddee3b233d/molcellb00111-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae4/370068/b33cdbcdb096/molcellb00111-0131-b.jpg

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引用本文的文献

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本文引用的文献

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Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.细胞质斑点杂交。对多个小细胞或组织样本中相对mRNA水平的简单分析。
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Chromatin conformational changes accompany transcriptional activation of a glucose-repressed gene in Saccharomyces cerevisiae.染色质构象变化伴随着酿酒酵母中一个葡萄糖抑制基因的转录激活。
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Estrogen induces a demethylation at the 5' end region of the chicken vitellogenin gene.雌激素可诱导鸡卵黄蛋白原基因5'端区域发生去甲基化。
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