Ehrlich R, Sharrow S O, Maguire J E, Singer D S
Experimental Immunology Branch, NIH, Bethesda, MD 20892.
Immunogenetics. 1989;30(1):18-26. doi: 10.1007/BF02421465.
Transgenic mice containing a swine class I major histocompatibility complex (MHC) gene, PD1, express swine MHC (SLA) antigen. The tissue distribution of PD1 RNA parallels that observed in the swine, indicating that the expression of PD1 is regulated and that trans-acting factors involved in this regulation have been conserved between the species. Although PD1 RNA levels were much greater in transgenic spleen than in thymus, no difference in the chromatin organization of the PD1 gene was detected. In both tissues, a single DNase I hypersensitive site mapped within the 5' flanking region. In vivo treatment of the transgenics with mouse alpha, beta-interferon increases PD1 expression in a number of tissues. In the spleen, this increase parallels that observed for the endogenous transplantation antigen, Kb, but differs markedly from the differentiation antigen, Qa-2. Increases in cell surface expression of both PD1 and Kb occurred equally in splenic T- and B-cell populations following alpha, beta-interferon treatment. In contrast, Qa-2 expression in B cells was enhanced by alpha, beta-interferon, whereas it was unaffected in T cells and thymocytes.
携带猪I类主要组织相容性复合体（MHC）基因PD1的转基因小鼠表达猪MHC（SLA）抗原。PD1 RNA的组织分布与在猪中观察到的相似，表明PD1的表达受到调控，并且参与这种调控的反式作用因子在物种间是保守的。尽管转基因小鼠脾脏中的PD1 RNA水平比胸腺中的高得多，但未检测到PD1基因染色质组织的差异。在这两种组织中，一个单一的DNase I超敏位点定位于5'侧翼区域。用小鼠α、β干扰素对转基因小鼠进行体内处理会增加许多组织中的PD1表达。在脾脏中，这种增加与内源性移植抗原Kb的情况相似，但与分化抗原Qa - 2明显不同。α、β干扰素处理后，脾脏T细胞和B细胞群体中PD1和Kb的细胞表面表达增加程度相同。相比之下，α、β干扰素增强了B细胞中Qa - 2的表达，而在T细胞和胸腺细胞中则未受影响。
