Fidelus R K, Laughter A H, Twomey J J
J Immunol. 1984 Mar;132(3):1462-5.
Polyamine synthesis occurs early in lymphocyte activation after stimulation with antigen or mitogen. Ornithine decarboxylase (ODC) is the primary enzyme in the polyamine cascade. We have examined the induction of ODC by mitogens and/or lymphokines in human peripheral blood T lymphocytes. When isolated populations of monocytes and T lymphocytes were stimulated with phytohemagglutinin (PHA) there was little or no change in ODC activity. The combination of T lymphocytes and monocytes enhanced mitogen-induced ODC activity 10-fold. Several interleukin 1 (IL 1)-containing supernatants and fractionated human IL 1 were capable of substituting for monocytes in supporting PHA induction of ODC in T lymphocytes. Interleukin 2 (IL 2) and IL 2-containing supernatants were also capable of increasing ODC activity in T lymphocytes in the absence of monocytes. Lymphokines alone in the absence of PHA could not induce ODC. We conclude that both mitogens and monocytes are required for the induction of polyamine synthesis in T lymphocytes, and that supernatants containing IL 1 or IL 1 and IL 2 can substitute for monocytes in the induction of ODC in mitogen-stimulated T lymphocytes.
在用抗原或有丝分裂原刺激后，多胺合成在淋巴细胞激活早期发生。鸟氨酸脱羧酶（ODC）是多胺级联反应中的主要酶。我们已经研究了有丝分裂原和/或淋巴因子对人外周血T淋巴细胞中ODC的诱导作用。当用植物血凝素（PHA）刺激分离的单核细胞和T淋巴细胞群体时，ODC活性几乎没有变化。T淋巴细胞和单核细胞的组合使有丝分裂原诱导的ODC活性增强了10倍。几种含白细胞介素1（IL-1）的上清液和纯化的人IL-1能够替代单核细胞，支持PHA诱导T淋巴细胞中的ODC。白细胞介素2（IL-2）和含IL-2的上清液在没有单核细胞的情况下也能够增加T淋巴细胞中的ODC活性。在没有PHA的情况下，单独的淋巴因子不能诱导ODC。我们得出结论，有丝分裂原和单核细胞都是T淋巴细胞中多胺合成诱导所必需的，并且含有IL-1或IL-1和IL-2的上清液可以在有丝分裂原刺激的T淋巴细胞中替代单核细胞诱导ODC。
