Boyer J, Sohaleh G, Frobert Y, Cordier J F, Touraine R
Rev Fr Mal Respir. 1983;11(6):889-98.
Cell typing of sub-populations of lymphocytes is facilitated by using monoclonal antibodies (OKT); with this technique we determined the relative proportions of "helper" T-lymphocytes (OKT4) and "Suppressor" T-lymphocytes (OKT8) in the blood and the LBA of 13 patients suffering from pulmonary sarcoidosis. In the control subjects the OKT4/OKT8 ratio was 1.9 +/- 0.3 in the blood (38 subjects) and of 2 +/- 0.2 in the LBA (6 subjects). Six patients suffering from inactive sarcoidosis (stabilised) had an OKT4/OKT8 ratio on average of 1.2 +/- 0.39 in the blood and of 1.86 +/- 0.46 in the LBA. By contrast in 7 patients suffering from "active" sarcoidosis, on average the ratio was 1.88 +/- 1.20 in the blood but 5.13 +/- 3.51 in the LBA; this ratio was notably raised in the LBA in 5 cases and was due as much to an increase in the T-"Helper" (OKT4) percentage as to a fall in the T-"Suppressor" (OKT8). It is probable that the T-"Helper" lymphocytes found in great numbers in the lung in active sarcoidosis cases participate in the formation of sarcoid granulomas and attract phagocytic mononuclear cells. This phenomenon seems to correspond to a local immune disturbance without producing a similar result in the peripheral blood of the patients.
使用单克隆抗体（OKT）有助于对淋巴细胞亚群进行细胞分型；通过这项技术，我们测定了13例肺结节病患者血液和支气管肺泡灌洗（LBA）中“辅助性”T淋巴细胞（OKT4）和“抑制性”T淋巴细胞（OKT8）的相对比例。在对照组中，血液中OKT4/OKT8比值为1.9±0.3（38例受试者），支气管肺泡灌洗中为2±0.2（6例受试者）。6例非活动性结节病（病情稳定）患者血液中OKT4/OKT8比值平均为1.2±0.39，支气管肺泡灌洗中为1.86±0.46。相比之下，7例“活动性”结节病患者血液中该比值平均为1.88±1.20，而支气管肺泡灌洗中为5.13±3.51；该比值在5例患者的支气管肺泡灌洗中显著升高，这同样是由于T“辅助性”（OKT4）百分比增加以及T“抑制性”（OKT8）百分比下降所致。在活动性结节病病例的肺中大量发现的T“辅助性”淋巴细胞可能参与了结节性肉芽肿的形成并吸引吞噬性单核细胞。这种现象似乎对应于局部免疫紊乱，而在患者外周血中未产生类似结果。
