2-巯基乙醇在自身免疫小鼠B细胞体外培养中诱导免疫球蛋白分泌细胞的产生。
Induction of immunoglobulin-secreting cells by 2-mercaptoethanol in in vitro culture of B cells from autoimmune mice.
作者信息
Fujiwara M, Kariyone A I
出版信息
Immunology. 1984 Apr;51(4):801-8.
Splenic B cells from older MRL/Mp-lpr/lpr (MRL/1) and male BXSB mice responded to 2-mercaptoethanol (2-ME) in in vitro culture and generated immunoglobulin-secreting cells (IgSC). Optimal concentration of 2-ME to induce IgSC was 5 X 10(-5) M. Kinetic studies revealed that the generation of IgSC was already apparent after 24 hr of culture and peak response was attained on the 2nd day. The response of B cells to 2 ME was enhanced in the presence of splenic T cells. Irradiation of B cells reduced the generation of IgSC. The B cell population of autoimmune mice responding to 2-ME to generate IgSC seems to be in a terminal stage of differentiation. This increased B cell differentiation was characteristic of autoimmune mice and assumed to have some significance in the development of autoimmune diseases.
来自老年MRL/Mp-lpr/lpr（MRL/1）和雄性BXSB小鼠的脾脏B细胞在体外培养中对2-巯基乙醇（2-ME）有反应，并产生分泌免疫球蛋白的细胞（IgSC）。诱导IgSC的2-ME最佳浓度为5×10⁻⁵M。动力学研究表明，培养24小时后IgSC的产生就已明显，第2天达到反应峰值。在脾脏T细胞存在的情况下，B细胞对2-ME的反应增强。B细胞经照射后IgSC的产生减少。对2-ME有反应以产生IgSC的自身免疫小鼠的B细胞群体似乎处于分化的终末阶段。这种B细胞分化增加是自身免疫小鼠的特征，被认为在自身免疫性疾病的发展中具有一定意义。
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