Ball E D, Guyre P M, Shen L, Glynn J M, Maliszewski C R, Baker P E, Fanger M W
J Clin Invest. 1984 Apr;73(4):1072-7. doi: 10.1172/JCI111292.
We investigated the ability of purified, recombinant DNA-derived interferons (IFN) to induce phenotypic changes in cells of the HL-60 promyelocytic leukemia cell line. Changes in cell surface markers detected by monoclonal antibodies as well as morphologic, histochemical, and functional changes were monitored. We found that gamma-IFN, but not alpha- or beta-IFN, induced the expression of antigens characteristic of monocytes and granulocytes (AML-2-23, 63D3, and 61D3), as well as changes in morphology consistent with monocytoid differentiation. These included induction of alpha-naphthyl acetate esterase, increased cell size, and a decrease in azurophilic granules. The gamma-IFN dose dependency and time course of the effect on antigen expression suggest that de novo protein synthesis was induced by gamma-IFN. The activity of gamma-IFN and of mixed-lymphocyte culture supernatant was blocked by a monoclonal antibody to gamma-IFN. Significant augmentation in the ability of the HL-60 cells to mediate antibody-dependent cellular cytotoxicity was induced by gamma-IFN. These findings suggest that gamma-IFN plays a role in the regulation of hematopoiesis.
我们研究了纯化的、重组DNA衍生的干扰素（IFN）诱导HL-60早幼粒细胞白血病细胞系细胞发生表型变化的能力。监测了单克隆抗体检测到的细胞表面标志物变化以及形态学、组织化学和功能变化。我们发现，γ-干扰素而非α-或β-干扰素可诱导单核细胞和粒细胞特征性抗原（AML-2-23、63D3和61D3）的表达，以及与单核细胞样分化一致的形态学变化。这些变化包括α-萘乙酸酯酶的诱导、细胞大小增加以及嗜天青颗粒减少。γ-干扰素对抗原表达影响的剂量依赖性和时间进程表明，γ-干扰素诱导了从头蛋白质合成。γ-干扰素的活性以及混合淋巴细胞培养上清液的活性被抗γ-干扰素单克隆抗体阻断。γ-干扰素可显著增强HL-60细胞介导抗体依赖性细胞毒性的能力。这些发现表明，γ-干扰素在造血调控中发挥作用。
